Pricing Availability   Qty
Description: Broad spectrum cysteine reactive probe
Chemical Name: N-5-Hexyn-1-yl-2-iodoacetamide
Purity: ≥97%

Biological Activity for IA-Alkyne

IA-Alkyne is a broad spectrum cysteine reactive probe. Covalently binds cysteine residues in proteins. Can be utilized for covalent fragment-based ligand discovery (FBLD) and to identify Zn2+-binding cysteines. Used as a click-chemistry handle to attach a fluorophore or biotin to reactive cysteines in proteins for visualization by in-gel fluorescence or identification by mass spectrometry, respectively. Covalently binds cysteine residues in and activates TRPA1 channels.

Technical Data for IA-Alkyne

M. Wt 265.09
Formula C8H12INO
Storage Store at -20°C
Purity ≥97%
CAS Number 930800-38-7
PubChem ID 44448710

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for IA-Alkyne

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 26.51 100
ethanol 26.51 100

Preparing Stock Solutions for IA-Alkyne

The following data is based on the product molecular weight 265.09. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.77 mL 18.86 mL 37.72 mL
5 mM 0.75 mL 3.77 mL 7.54 mL
10 mM 0.38 mL 1.89 mL 3.77 mL
50 mM 0.08 mL 0.38 mL 0.75 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for IA-Alkyne

References are publications that support the biological activity of the product.

Backus et al (2016) Proteome-wide covalent ligand discovery in native biological systems. Nature 534 570 PMID: 27309814

Pace and Weerapana et al (2014) A competitive chemical-proteomic platform to identify zinc-binding cysteines. ACS Chem.Biol. 9 258 PMID: 24111988

Macpherson et al (2007) Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature 445 541 PMID: 17237762

If you know of a relevant reference for IA-Alkyne, please let us know.

View Related Products by Target

Keywords: IA-Alkyne, IA-Alkyne supplier, indoacetamide, IAA, cysteines, reactive, probe, covalent, binding, binds, covalently, fragment-based, ligand, discovery, FBLD, iso-TOP-ABPP, isotopic, tandem, orthogonal, proteolysis, activity, based, protein, profiling, Biochemicals, and, Molecular, Biology, 7015, Tocris Bioscience

Citations for IA-Alkyne

Citations are publications that use Tocris products.

Currently there are no citations for IA-Alkyne. Do you know of a great paper that uses IA-Alkyne from Tocris? Please let us know.

Reviews for IA-Alkyne

There are currently no reviews for this product. Be the first to review IA-Alkyne and earn rewards!

Have you used IA-Alkyne?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review