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Description: Adrenal glucocorticoid; immunosuppressant
Chemical Name: 11β,17α,21-Trihydroxypregn-4-ene-3,20-dione
Purity: ≥98% (HPLC)
Citations (4)
Reviews (1)
Literature (1)

Biological Activity for Hydrocortisone

Hydrocortisone is an adrenal glucocorticoid released in the stress response. Increases glucose production by gluconeogenesis. Immunosuppressant and antidiuretic hormone. Used in protocols to generate retinal pigment epithelial (RPE) cells from PSCs.

Compound Libraries for Hydrocortisone

Hydrocortisone is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Hydrocortisone

M. Wt 362.46
Formula C21H30O5
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 50-23-7
PubChem ID 5754
Smiles O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@@]([H])2[C@@H](O)C[C@@]4(C)[C@]([H])3CC[C@@](O)4[C@@](CO)=O)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Hydrocortisone

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 36.25 100
ethanol 9.06 25

Preparing Stock Solutions for Hydrocortisone

The following data is based on the product molecular weight 362.46. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.76 mL 13.79 mL 27.59 mL
5 mM 0.55 mL 2.76 mL 5.52 mL
10 mM 0.28 mL 1.38 mL 2.76 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

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Product Datasheets for Hydrocortisone

Certificate of Analysis / Product Datasheet
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References for Hydrocortisone

References are publications that support the biological activity of the product.

Khani and Tayek (2001) Cortisol increases gluconeogenesis in humans; its role in the metabolic syndrome. Clin.Sci.(Lond) 101 739 PMID: 11724664

Surendran et al (2022) An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells STAR Protoc. 3 101803 PMID: 36386870

If you know of a relevant reference for Hydrocortisone, please let us know.

Keywords: Hydrocortisone, Hydrocortisone supplier, glucocorticoids, antiinflammatory, stress, induces, inducers, gluconeogenesis, adrenal, gland, 11b,17a,21-Trihydroxypregn-4-ene-3,20-dione, Glucocorticoid, Receptor, Immunosuppressants, Retinal, Stem, Cells, 4093, Tocris Bioscience

4 Citations for Hydrocortisone

Citations are publications that use Tocris products. Selected citations for Hydrocortisone include:

Frost et al (2017) The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo. Methods Enzymol 587 43 PMID: 28253971

Srinivasan et al (2018) Androgen receptor expression reduces stemness characteristics of prostate cancer cells (PC3) by repression of CD44 and SOX2. J Cell Biochem PMID: 30206982

Paul F et al (2021) Cell Populations Expressing Stemness-Associated Markers in Lung Adenocarcinoma. Life (Basel) 11 PMID: 34685477

Georges et al (2022) Polyploid giant cancer cells, EZH2 and Myc upregulation in mammary epithelial cells infected with high-risk human cytomegalovirus. EBioMedicine 80 104056 PMID: 35596973

Do you know of a great paper that uses Hydrocortisone from Tocris? Please let us know.

Reviews for Hydrocortisone

Average Rating: 4 (Based on 1 Review.)

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cell culture.
By Irving Garcia on 11/19/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: ES cells

Used before always use from Tocris. Always works well in cell culture.

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Depression Poster

Depression Poster

Major depressive disorder is characterized by depressed mood and a loss of interest and/or pleasure. Updated in 2015 this poster highlights presynaptic and postsynaptic targets for the potential treatment of major depressive disorder, as well as outlining the pharmacology of currently approved antidepressant drugs.