Hoechst 33258

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Cat.No. 5824 - Hoechst 33258 | C25H24N6O.3HCl | CAS No. 23491-45-4
Description: Fluorescent dye for labeling DNA
Chemical Name: 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.

Technical Data

M. Wt 533.88
Formula C25H24N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 23491-45-4
PubChem ID 5360743
InChI Key OKQAASMPIOSOEX-UHFFFAOYSA-N
Smiles CN(CC1)CCN1C2=CC(N=C(C3=CC(NC(C4=CC=C(O)C=C4)=N5)=C5C=C3)N6)=C6C=C2.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.68 20
water 53.39 100

Preparing Stock Solutions

The following data is based on the product molecular weight 533.88. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.87 mL 9.37 mL 18.73 mL
5 mM 0.37 mL 1.87 mL 3.75 mL
10 mM 0.19 mL 0.94 mL 1.87 mL
50 mM 0.04 mL 0.19 mL 0.37 mL

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References

References are publications that support the biological activity of the product.

Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089

Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559


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