Hoechst 33258

Discontinued Product

Hoechst 33258 (Cat. No. 5824) has been withdrawn from sale for commercial reasons.
Cat.No. 5824 - Hoechst 33258 | C25H24N6O.3HCl | CAS No. 23491-45-4
Description: Fluorescent dye for staining DNA
Chemical Name: 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews
Literature (1)

Biological Activity for Hoechst 33258

Hoechst 33258 is a fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.

Optical Data for Hoechst 33258

λabs 352 nm
λem 461 nm
Application Flow Cytometry

Technical Data for Hoechst 33258

M. Wt 533.88
Formula C25H24N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 23491-45-4
PubChem ID 5360743
InChI Key OKQAASMPIOSOEX-UHFFFAOYSA-N
Smiles CN(CC1)CCN1C2=CC(N=C(C3=CC(NC(C4=CC=C(O)C=C4)=N5)=C5C=C3)N6)=C6C=C2.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Hoechst 33258

References for Hoechst 33258

References are publications that support the biological activity of the product.

Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089

Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559

Keywords: Hoechst 33258, Hoechst 33258 supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5824, Tocris Bioscience

2 Citations for Hoechst 33258

Citations are publications that use Tocris products. Selected citations for Hoechst 33258 include:

Moreno et al (2019) Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity. Life Sci Alliance 2 PMID: 30979826

Terheyden-Keighley et al (2018) CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro. Biol Open 7 PMID: 30135081


Reviews for Hoechst 33258

There are currently no reviews for this product. Be the first to review Hoechst 33258 and earn rewards!

Have you used Hoechst 33258?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals