Fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 533.88. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.87 mL||9.37 mL||18.73 mL|
|5 mM||0.37 mL||1.87 mL||3.75 mL|
|10 mM||0.19 mL||0.94 mL||1.87 mL|
|50 mM||0.04 mL||0.19 mL||0.37 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089
Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 461559
If you know of a relevant reference for Hoechst 33258, please let us know.
Keywords: Hoechst 33258, supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Dyes, Tocris Bioscience
Citations for Hoechst 33258
Citations are publications that use Tocris products.
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Literature in this Area
New Product Guide (Spring/Summer 2017)New
Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.
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