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Fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.
Optical Data for Hoechst 33258
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 533.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.87 mL||9.37 mL||18.73 mL|
|5 mM||0.37 mL||1.87 mL||3.75 mL|
|10 mM||0.19 mL||0.94 mL||1.87 mL|
|50 mM||0.04 mL||0.19 mL||0.37 mL|
References are publications that support the biological activity of the product.
Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089
Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559
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Keywords: Hoechst 33258, Hoechst 33258 supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5824, Tocris Bioscience
2 Citations for Hoechst 33258
Citations are publications that use Tocris products. Selected citations for Hoechst 33258 include:
Moreno et al (2019) Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity. Life Sci Alliance 2 PMID: 30979826
Terheyden-Keighley et al (2018) CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro. Biol Open 7 PMID: 30135081
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Literature in this Area
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