Hoechst 33258

Discontinued Product

Hoechst 33258 (Cat. No. 5824) has been withdrawn from sale for commercial reasons.
Description: Fluorescent dye for staining DNA
Chemical Name: 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
Purity: ≥98% (HPLC)
Citations (2)
Literature (1)

Biological Activity for Hoechst 33258

Hoechst 33258 is a fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.

Optical Data for Hoechst 33258

λabs 352 nm
λem 461 nm
Application Flow Cytometry

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Technical Data for Hoechst 33258

M. Wt 533.88
Formula C25H24N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 23491-45-4
PubChem ID 5360743
Smiles CN(CC1)CCN1C2=CC(N=C(C3=CC(NC(C4=CC=C(O)C=C4)=N5)=C5C=C3)N6)=C6C=C2.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Hoechst 33258

References for Hoechst 33258

References are publications that support the biological activity of the product.

Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089

Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559

Keywords: Hoechst 33258, Hoechst 33258 supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5824, Tocris Bioscience

2 Citations for Hoechst 33258

Citations are publications that use Tocris products. Selected citations for Hoechst 33258 include:

Moreno et al (2019) Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity. Life Sci Alliance 2 PMID: 30979826

Terheyden-Keighley et al (2018) CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro. Biol Open 7 PMID: 30135081

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals