Hoechst 33258

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Cat.No. 5824 - Hoechst 33258 | C25H24N6O.3HCl | CAS No. 23491-45-4
Description: Fluorescent dye for staining DNA
Chemical Name: 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
Purity: ≥98% (HPLC)
Citations (2)
Literature (1)

Biological Activity

Fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.

Optical Data for Hoechst 33258

λabs 352 nm
λem 461 nm
Application Flow Cytometry

Technical Data

M. Wt 533.88
Formula C25H24N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 23491-45-4
PubChem ID 5360743
Smiles CN(CC1)CCN1C2=CC(N=C(C3=CC(NC(C4=CC=C(O)C=C4)=N5)=C5C=C3)N6)=C6C=C2.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 10.68 20
water 53.39 100

Preparing Stock Solutions

The following data is based on the product molecular weight 533.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.87 mL 9.37 mL 18.73 mL
5 mM 0.37 mL 1.87 mL 3.75 mL
10 mM 0.19 mL 0.94 mL 1.87 mL
50 mM 0.04 mL 0.19 mL 0.37 mL

Molarity Calculator

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References are publications that support the biological activity of the product.

Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089

Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559

If you know of a relevant reference for Hoechst 33258, please let us know.

Keywords: Hoechst 33258, Hoechst 33258 supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5824, Tocris Bioscience

2 Citations for Hoechst 33258

Citations are publications that use Tocris products. Selected citations for Hoechst 33258 include:

Moreno et al (2019) Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity. Life Sci Alliance 2 PMID: 30979826

Terheyden-Keighley et al (2018) CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro. Biol Open 7 PMID: 30135081

Do you know of a great paper that uses Hoechst 33258 from Tocris? Please let us know.

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Literature in this Area

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Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

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