Hoechst 33258

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Cat.No. 5824 - Hoechst 33258 | C25H24N6O.3HCl | CAS No. 23491-45-4
Description: Fluorescent dye for labeling DNA
Chemical Name: 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.

Technical Data

M. Wt 533.88
Formula C25H24N6O.3HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 23491-45-4
PubChem ID 5360743
InChI Key OKQAASMPIOSOEX-UHFFFAOYSA-N
Smiles CN(CC1)CCN1C2=CC(N=C(C3=CC(NC(C4=CC=C(O)C=C4)=N5)=C5C=C3)N6)=C6C=C2.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.68 20
water 53.39 100

Preparing Stock Solutions

The following data is based on the product molecular weight 533.88. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.87 mL 9.37 mL 18.73 mL
5 mM 0.37 mL 1.87 mL 3.75 mL
10 mM 0.19 mL 0.94 mL 1.87 mL
50 mM 0.04 mL 0.19 mL 0.37 mL

Molarity Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089

Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 461559


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Keywords: Hoechst 33258, supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Dyes, Tocris Bioscience

Citations for Hoechst 33258

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New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for Hoechst 33258

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