Fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 533.88. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.87 mL||9.37 mL||18.73 mL|
|5 mM||0.37 mL||1.87 mL||3.75 mL|
|10 mM||0.19 mL||0.94 mL||1.87 mL|
|50 mM||0.04 mL||0.19 mL||0.37 mL|
References are publications that support the biological activity of the product.
Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089
Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559
If you know of a relevant reference for Hoechst 33258, please let us know.
Keywords: Hoechst 33258, Hoechst 33258 supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Stains, 5824, Tocris Bioscience
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