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Description: Fluorescent NAD+; substrate for ADP-ribosylation for use in PARP assays
Chemical Name: β-Nicotinamide-N6-[2-[[6-[fluorescein]-amino]-2-oxohexyl-hexyl]amino]-2-oxoethyl]adenine dinucleotide
Literature (1)

Biological Activity for Fluorescein-NAD+

Fluorescein-NAD+ is a fluoroscein NAD+ is a substrate for ADP-ribosylation, providing a convenient non-isotopic alternative to radiolabelled NAD for use in PARP assays. Allows direct measurement of NAD-dependent enzymes, such as PARP, by fluorescence microscopy. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.

This product is a replacement for R&D Systems product 4673-500-01

81 μg is supplied as 250 μl of a 0.25 mM solution in water.

Technical Data for Fluorescein-NAD+

M. Wt 1291.11
Formula C56H64N10O22P2
Storage Store at -80°C
Smiles O=C(CCCCCNC(C1=CC(C(O)=O)=C(C2=C(C=C3)C(OC4=C2C=CC(O)=C4)=CC3=O)C=C1)=O)NCCCCCCNC(CNC5=C(N=CN6[C@@H]7O[C@@H]([C@H]([C@H]7O)O)COP(O)(OP(OC[C@H]8O[C@@H]([N+]9=CC=CC(C(N)=O)=C9)[C@H](O)[C@@H]8O)([O-])=O)=O)C6=NC=N5)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Fluorescein-NAD+

Solubility Soluble in water (supplied pre-dissolved at a concentration of 0.25mM)

Product Datasheets for Fluorescein-NAD+

Certificate of Analysis / Product Datasheet
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References for Fluorescein-NAD+

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Keywords: Fluorescein-NAD+, Fluorescein-NAD+ supplier, Fluorescein-NAD-Plus, fluorescent, PARP, substrate, assay, TR-FRET, acceptor, dyes, Biochemicals, and, Molecular, Biology, Fluorescent, Substrates, Enzyme, Probes, FP, Assay, Reagents, 6574, Tocris Bioscience

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Literature in this Area

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