Home / Product Type / Fluorescence Imaging / Fluorescent Probes and Stains / Calcium Signaling Probes and Ca2+ Indicator Dyes / Fluo-4 AM

Fluo-4 AM

Cat. No. 6255 2 Reviews Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Cat.No. 6255 - Fluo-4 AM | C51H50F2N2O23 | CAS No. 273221-67-3
Description: Cell-permeable, fluorescent Ca2+ indicator
Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews (2)
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.

Technical Data

M. Wt 1096.95
Formula C51H50F2N2O23
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 273221-67-3
PubChem ID 4060965
InChI Key QOMNQGZXFYNBNG-UHFFFAOYSA-N
Smiles O=C(C(F)=C1)C=C2C1=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)C5=CC(F)=C(OCOC(C)=O)C=C5O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.1 1

Preparing Stock Solutions

The following data is based on the product molecular weight 1096.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 91.16 mL 455.81 mL 911.62 mL
0.05 mM 18.23 mL 91.16 mL 182.32 mL
0.1 mM 9.12 mL 45.58 mL 91.16 mL
0.5 mM 1.82 mL 9.12 mL 18.23 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the biological activity of the product.

Gee et al (2000) Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes. Cell Calcium. 27 97 PMID: 10756976

If you know of a relevant reference for Fluo-4 AM, please let us know.

View Related Products by Target

Keywords: Fluo-4 AM, Fluo-4 AM supplier, Fluo-4AM, Fluo-4, cell, permeable, calcium, Ca2+, indicator, fluorescent, Ion, Indicators, General, Calcium, Signaling, Agents, Probes, and, Indicator, Dyes, 6255, Tocris Bioscience

Citations for Fluo-4 AM

Citations are publications that use Tocris products.

Currently there are no citations for Fluo-4 AM. Do you know of a great paper that uses Fluo-4 AM from Tocris? Please let us know.

Reviews for Fluo-4 AM

Average Rating: 4.5 (Based on 2 Reviews.)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Fluo-4 AM?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells..
By Niroj Shrestha on 12/24/2019
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: RBL-2H3

RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.

review image
works well in motor neurons.
By Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: motor neurons

Used for imaging calcium entry in motor neurons at 1 mM concentration

motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals
Ion Channel

Ion Channel Product Listing

A collection of around 500 products for ion channel research, the listing includes research tools for the study of:

  • Ligand-gated ion channels
  • Voltage-gated ion channels
  • Other Ion Channels