Fluo-4 AM

Pricing Availability   Qty
Description: Cell-permeable, fluorescent Ca2+ indicator
Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Citations (2)
Reviews (2)
Literature (1)

Biological Activity for Fluo-4 AM

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Fluo-4 AM

Emission Color Green
λabs 494 nm
λem 506 nm
Closest Laser line 488 nm
Cell Permeable Yes
Application Fluorescence Microscopy, Flow Cytometry, Fluorometric Microplate Reading Assays

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Fluo-4 AM

M. Wt 1096.95
Formula C51H50F2N2O23
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 273221-67-3
PubChem ID 4060965

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Fluo-4 AM

Certificate of Analysis / Product Datasheet
Select another batch:

References for Fluo-4 AM

References are publications that support the biological activity of the product.

Gee et al (2000) Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes. Cell Calcium. 27 97 PMID: 10756976

If you know of a relevant reference for Fluo-4 AM, please let us know.

Keywords: Fluo-4 AM, Fluo-4 AM supplier, Fluo-4AM, Fluo-4, cell, permeable, calcium, Ca2+, indicator, fluorescent, Ion, Indicators, General, Calcium, Signaling, Agents, Fluorescent, 6255, Tocris Bioscience

2 Citations for Fluo-4 AM

Citations are publications that use Tocris products. Selected citations for Fluo-4 AM include:

Gerald W et al (2022) Norepinephrine transporter defects lead to sympathetic hyperactivity in Familial Dysautonomia models. Nat Commun 13 7032 PMID: 36396637

Bernardo et al (2020) Heterosynaptic Plasticity Determines the Set Point for Cortical Excitatory-Inhibitory Balance. Neuron 106 842-854.e4 PMID: 32213321

Do you know of a great paper that uses Fluo-4 AM from Tocris? Please let us know.

Reviews for Fluo-4 AM

Average Rating: 4.5 (Based on 2 Reviews.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Fluo-4 AM?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells..
By Niroj Shrestha on 12/24/2019
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: RBL-2H3

RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.

review image

works well in motor neurons.
By Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: motor neurons

Used for imaging calcium entry in motor neurons at 1 mM concentration

motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals