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Biological Activity for Fluo-4 AM
Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.
Technical Data for Fluo-4 AM
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Fluo-4 AM
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Fluo-4 AM
The following data is based on the product molecular weight 1096.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.01 mM||91.16 mL||455.81 mL||911.62 mL|
|0.05 mM||18.23 mL||91.16 mL||182.32 mL|
|0.1 mM||9.12 mL||45.58 mL||91.16 mL|
|0.5 mM||1.82 mL||9.12 mL||18.23 mL|
References for Fluo-4 AM
References are publications that support the biological activity of the product.
Gee et al (2000) Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes. Cell Calcium. 27 97 PMID: 10756976
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Citations for Fluo-4 AM
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Reviews for Fluo-4 AM
Average Rating: 4.5 (Based on 2 Reviews.)
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RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
Used for imaging calcium entry in motor neurons at 1 mM concentration
motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.
Literature in this Area
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