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Submit ReviewFluo-4 AM
Description: Cell-permeable, fluorescent Ca2+ indicator
Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Biological Activity
Cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.
Technical Data
| M. Wt | 1096.95 |
| Formula | C51H50F2N2O23 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 273221-67-3 |
| PubChem ID | 4060965 |
| InChI Key | QOMNQGZXFYNBNG-UHFFFAOYSA-N |
| Smiles | O=C(C(F)=C1)C=C2C1=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)C5=CC(F)=C(OCOC(C)=O)C=C5O2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 1.1 | 1 |
Preparing Stock Solutions
The following data is based on the product molecular weight 1096.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 91.16 mL | 455.81 mL | 911.62 mL |
| 0.05 mM | 18.23 mL | 91.16 mL | 182.32 mL |
| 0.1 mM | 9.12 mL | 45.58 mL | 91.16 mL |
| 0.5 mM | 1.82 mL | 9.12 mL | 18.23 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Gee et al (2000) Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes. Cell Calcium. 27 97 PMID: 10756976
If you know of a relevant reference for Fluo-4 AM, please let us know.
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Keywords: Fluo-4 AM, Fluo-4 AM supplier, Fluo-4AM, Fluo-4, cell, permeable, calcium, Ca2+, indicator, fluorescent, Ion, Indicators, General, Calcium, Signaling, Agents, 6255, Tocris Bioscience
Citations for Fluo-4 AM
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Reviews for Fluo-4 AM
Average Rating: 5 (Based on 1 Review.)
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works well in motor neurons.
By
Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: motor neurons
Used for imaging calcium entry in motor neurons at 1 mM concentration
motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.
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