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Biological Activity for FFN 270
FFN 270 is a fluorescent false neurotransmitter (FFN). Fluorescent substrate for NET and VMAT2. Labels noradrenergic neurons and their synaptic vesicles, and enables imaging of synaptic vesicle content release from specific axonal sites in vivo. Selectively labels NA neurons over other monoamine and CNS targets. Exhibits two resolved absorption/excitation maxima depending on solvent pH (excitation maxima: 320 nm or 365 nm, emission maxima: 475 nm).
Sold with the permission of Columbia University.
Amphetamine-stimulated release of FFN 270 from noradrenergic projections for FFN 270
Amphetamine releases FFN 270 from noradrenergic projections in vivo. The video shows FFN 270 puncta in Layer 1 of the barrel cortex in mice taken before and after injection of amphetamine (AMPH).
Technical Data for FFN 270
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FFN 270
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for FFN 270
The following data is based on the product molecular weight 259.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.85 mL||19.26 mL||38.51 mL|
|5 mM||0.77 mL||3.85 mL||7.7 mL|
|10 mM||0.39 mL||1.93 mL||3.85 mL|
|50 mM||0.08 mL||0.39 mL||0.77 mL|
References for FFN 270
References are publications that support the biological activity of the product.
Dunn et al (2018) Designing a norepinephrine optical tracer for imaging individual noradrenergic synapses and their activity in vivo. Nat.Commun. 9 2838 PMID: 30026491
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Citations for FFN 270
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Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals