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FFN 102 mesylate
pH responsive fluorescent false neurotransmitter (FFN). Selective dopamine transporter (DAT) and VMAT2 substrate. Exhibits no significant binding to a panel of 38 CNS receptors, including dopamine and serotonin receptors. Inhibits dopamine uptake. Excitation maxima are 340 nm at pH 5 and 370 nm at pH 7.5. Emission maximum is 435 nm at both pH 5 and 7.5.
Sold with the permission of Columbia University.
|Storage||Desiccate at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|water||6.72||20 with gentle warming|
Preparing Stock Solutions
The following data is based on the product molecular weight 335.76. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.98 mL||14.89 mL||29.78 mL|
|5 mM||0.6 mL||2.98 mL||5.96 mL|
|10 mM||0.3 mL||1.49 mL||2.98 mL|
|50 mM||0.06 mL||0.3 mL||0.6 mL|
References are publications that support the biological activity of the product.
Rodriguez et al (2013) Fluorescent DA tracer resolves individual DArgic synapses and their activity in the brain. Proc.Natl.Acad.Sci.U.S.A. 110 870 PMID: 23277566
Sames et al (2013) Visualizing neurotransmitter secretion at individual synapses. ACS Chem.Neurosci. 4 648 PMID: 23862751
Lee et al (2010) Development of pH-responsive fluorescent false neurotransmitters. J.Am.Chem.Soc. 132 8828 PMID: 20540519
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