FAM-DEALA-Hyp-YIPD

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Cat.No. 7287 - FAM-DEALA-Hyp-YIPD | FAM-Asp-Glu-Ala-Leu-Ala-Hyp-Tyr-Ile-Pro-Asp
Description: Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (4)

Biological Activity

Fluorescent HIF-1α peptide (Kd = 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm.

Optical Data for FAM-DEALA-Hyp-YIPD

λabs 485 nm
λem 520 nm
Closest Laser line 488 nm
Application Fluorescence polarization (FP) displacement assays

Technical Data

M. Wt 1477.48
Formula C71H84N10O25
Sequence DEALAXYIPD

(Modifications: Asp-1 = (5,6)-FAM-Asp, X-6 = Hyp)

Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key CWNCTVVEODBMBK-RHNKRQRVSA-N
Smiles O=C([C@@H]1CCCN1C([C@H]([C@@H](CC)C)NC([C@H](CC2=CC=C(O)C=C2)NC([C@@H]3C[C@@H](O)CN3C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C4=CC(C(O5)=O)=C(C=C4)C65C7=CC=C(O)C=C7OC8=C6C=CC(O)=C8)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O.O=C([C@@H]9CCCN9C([C@H]([C@@H](CC)C)NC([C@H](CC%10=CC=C(O)C=C%10)NC([C@@H]%11C[C@@H](O)CN%11C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C(C=C%12)=CC(C%13%14C%15=CC=C(O)C=C%15OC%16=C%13C=CC(O)=C%16)=C%12C(O%14)=O)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solubility Soluble to 1 mg/ml in 0.01M PBS

References

References are publications that support the biological activity of the product.

Buckley et al (2012) Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction J.Am.Chem.Soc. 134 4465 PMID: 22369643

Lucas et al (2018) Surface probing by fragment-based screening and computational methods identifies ligandable pockets on the von Hippel-Lindau (VHL) E3 ubiquitin ligase J.Med.Chem. 61 7387 PMID: 30040896

Roy et al (2019) SPR-measured kinetics of PROTAC ternary complexes influence target degradation rate. ACS Chem.Biol. 14 361 PMID: 30721025

Crew et al (2018) Identification and Characterization of Von Hippel-Lindau-Recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-Binding Kinase 1 J.Med.Chem. 61 583 PMID: 28692295

Ahn et al (2009) HIF-1alpha peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1alpha Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556


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Keywords: FAM-DEALA-Hyp-YIPD, FAM-DEALA-Hyp-YIPD supplier, Fluorescent, HIF-1α, peptides, Fluorescence, polarization, (FP), displacement, assays, hypoxia, inducible, factor, HIF, pathways, Other, Probes, Hypoxia, Inducible, Factors, Active, Degraders, 7287, Tocris Bioscience

Citations for FAM-DEALA-Hyp-YIPD

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Literature in this Area

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