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Submit ReviewFAM-DEALA-Hyp-YIPD
Description: Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Purity: ≥95% (HPLC)
Biological Activity for FAM-DEALA-Hyp-YIPD
FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide (Kd = 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm. Longer peptide version, FAM-DEALAHypYIPMDDDFQLRSF (Cat. No. 7452), also available.
Optical Data for FAM-DEALA-Hyp-YIPD
| λabs | 485 nm |
|---|---|
| λem | 520 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence polarization (FP) displacement assays |
Technical Data for FAM-DEALA-Hyp-YIPD
| M. Wt | 1477.48 |
| Formula | C71H84N10O25 |
| Sequence |
DEALAXYIPD (Modifications: Asp-1 = (5,6)-FAM-Asp, X-6 = Hyp) |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | CWNCTVVEODBMBK-RHNKRQRVSA-N |
| Smiles | O=C([C@@H]1CCCN1C([C@H]([C@@H](CC)C)NC([C@H](CC2=CC=C(O)C=C2)NC([C@@H]3C[C@@H](O)CN3C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C4=CC(C(O5)=O)=C(C=C4)C65C7=CC=C(O)C=C7OC8=C6C=CC(O)=C8)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O.O=C([C@@H]9CCCN9C([C@H]([C@@H](CC)C)NC([C@H](CC%10=CC=C(O)C=C%10)NC([C@@H]%11C[C@@H](O)CN%11C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C(C=C%12)=CC(C%13%14C%15=CC=C(O)C=C%15OC%16=C%13C=CC(O)=C%16)=C%12C(O%14)=O)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FAM-DEALA-Hyp-YIPD
| Solubility | Soluble to 1 mg/ml in 0.01M PBS |
Product Datasheets for FAM-DEALA-Hyp-YIPD
References for FAM-DEALA-Hyp-YIPD
References are publications that support the biological activity of the product.
Buckley et al (2012) Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 134 4465 PMID: 22369643
Lucas et al (2018) Surface probing by fragment-based screening and computational methods identifies ligandable pockets on the von Hippel-Lindau (VHL) E3 ubiquitin ligase. J.Med.Chem. 61 7387 PMID: 30040896
Roy et al (2019) SPR-measured kinetics of PROTAC ternary complexes influence target degradation rate. ACS Chem.Biol. 14 361 PMID: 30721025
Crews et al (2018) Identification and characterization of von Hippel-Lindau-recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-binding kinase 1. J.Med.Chem. 61 583 PMID: 28692295
Ahn et al (2009) HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556
If you know of a relevant reference for FAM-DEALA-Hyp-YIPD, please let us know.
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Keywords: FAM-DEALA-Hyp-YIPD, FAM-DEALA-Hyp-YIPD supplier, Fluorescent, HIF-1α, alfa, alpha, peptides, Fluorescence, polarization, (FP), displacement, assays, hypoxia, inducible, factor, HIF, pathways, VHL, von, Hippel-Lindau, Hypoxia, Inducible, Factors, Enzyme, Probes, and, Substrates, Ubiquitin, E3, Ligases, 7287, Tocris Bioscience
Citations for FAM-DEALA-Hyp-YIPD
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