FAM-DEALAHypYIPMDDDFQLRSF

Pricing Availability   Qty
Cat.No. 7452 - FAM-DEALAHypYIPMDDDFQLRSF | FAM-Asp-Glu-Ala-Leu-Ala-Hyp-Tyr-Ile-Pro-Met-Asp-Asp-Asp-Phe-Gln-Leu-Arg-Ser-Phe-NH2
Description: Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (3)

Biological Activity for FAM-DEALAHypYIPMDDDFQLRSF

FAM-DEALAHypYIPMDDDFQLRSF is a 5,6-carboxyfluorescein (FAM) labeled HIF-1α peptide. It binds with high affinity to von Hippel-Lindau (VHL) protein (KD = 3 nM). Can be used to assess ligand binding to VHL in a direct fluorescence polarization (FP) displacement assay. Excitation/emission maxima (λ) = 485/520 nm. This product is a longer peptide version of FAM-DEALA-Hyp-YIPD (Cat. No. 7287).

Optical Data for FAM-DEALAHypYIPMDDDFQLRSF

Emission Color Green
λabs 485 nm
λem 520 nm
Closest Laser line 488 nm
Cell Permeable Yes
Application FP assay to determine VHL binding affinity, useful for PROTAC/Degrader development
Alternative For 7287

Technical Data for FAM-DEALAHypYIPMDDDFQLRSF

M. Wt 2616.76
Formula C122H158N24O39S
Sequence DEALAXYIPMDDDFQLRSF

(Modifications: Asp-1 = (5,6)-FAM-Asp, X-6 = Hyp, Phe-19 = C-terminal amide)

Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key BWRLQGDMCXAUCD-GMYGBYBYSA-N
Smiles O=C(C1=CC(C(O2)=O)=C(C=C1)C32C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N6[C@@H](CC[C@H]6O)C(N[C@@H](CC7=CC=C(C=C7)O)C(N[C@@H]([C@@H](C)CC)C(N8[C@@H](CCC8)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC9=CC=CC=C9)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%10=CC=CC=C%10)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.O=C(C(C=C%11)=CC(C%12%13C%14=CC=C(O)C=C%14OC%15=C%12C=CC(O)=C%15)=C%11C(O%13)=O)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N%16[C@@H](CC[C@H]%16O)C(N[C@@H](CC%17=CC=C(C=C%17)O)C(N[C@@H]([C@@H](C)CC)C(N%18[C@@H](CCC%18)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC%19=CC=CC=C%19)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%20=CC=CC=C%20)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FAM-DEALAHypYIPMDDDFQLRSF

Solubility Soluble to 1 mg/ml in PBS (pH 7.4)

Product Datasheets for FAM-DEALAHypYIPMDDDFQLRSF

References for FAM-DEALAHypYIPMDDDFQLRSF

References are publications that support the biological activity of the product.

Ahn et al (2009) HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556

Buckley et al (2012) Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 134 4465 PMID: 22369643

Crews et al (2018) Identification and Characterization of Von Hippel-Lindau-Recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-Binding Kinase 1. J.Med.Chem. 61 583 PMID: 28692295


If you know of a relevant reference for FAM-DEALAHypYIPMDDDFQLRSF, please let us know.

Keywords: FAM-DEALAHypYIPMDDDFQLRSF, FAM-DEALAHypYIPMDDDFQLRSF supplier, Fluorescent, HIF-1α, alpha, alfa, peptides, Fluorescence, polarization, (FP), displacement, assays, hypoxia, inducible, factor, HIF, VHL, von, Hippel-Lindau, Ubiquitin, E3, Ligases, Hypoxia, Inducible, Factors, Enzyme, Probes, and, Substrates, 7452, Tocris Bioscience

Citations for FAM-DEALAHypYIPMDDDFQLRSF

Citations are publications that use Tocris products.

Currently there are no citations for FAM-DEALAHypYIPMDDDFQLRSF. Do you know of a great paper that uses FAM-DEALAHypYIPMDDDFQLRSF from Tocris? Please let us know.

Reviews for FAM-DEALAHypYIPMDDDFQLRSF

There are currently no reviews for this product. Be the first to review FAM-DEALAHypYIPMDDDFQLRSF and earn rewards!

Have you used FAM-DEALAHypYIPMDDDFQLRSF?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Targeted Protein Degradation

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders
  • Degrader Building Blocks
  • Custom Degrader Services
  • UPS Proteins and Assays
  • Assays for Protein Degradation
Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Targeted Protein Degradation

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia