eCF 309

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Cat.No. 5955 - eCF 309 | C18H21N7O3 | CAS No. 2001571-40-8
Description: Potent mTOR inhibitor
Chemical Name: 3-(2-Amino-5-benzoxazolyl)-1-(2,2-diethoxyethyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
Purity: ≥98% (HPLC)
Literature (3)
Pathways (1)

Biological Activity for eCF 309

eCF 309 is a potent mTOR inhibitor (IC50 = 15 nM). Exhibits >60-fold selectivity for mTOR over PI 3-kinases and is inactive against a panel of other kinases. Inhibits growth of ER-positive MCF7 breast cancer cells in vitro (EC50 = 8.5 nM). Cell permeable. is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of eCF 309 is reviewed on the Chemical Probes website.

Technical Data for eCF 309

M. Wt 383.41
Formula C18H21N7O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2001571-40-8
PubChem ID 122163152
Smiles CCOC(OCC)CN1N=C(C2=CC(N=C(N)O3)=C3C=C2)C4=C1N=CN=C4N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for eCF 309

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 7.67 20

Preparing Stock Solutions for eCF 309

The following data is based on the product molecular weight 383.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 13.04 mL 65.2 mL 130.41 mL
1 mM 2.61 mL 13.04 mL 26.08 mL
2 mM 1.3 mL 6.52 mL 13.04 mL
10 mM 0.26 mL 1.3 mL 2.61 mL

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References for eCF 309

References are publications that support the biological activity of the product.

Fraser et al (2016) eCF309: a potent, selective and cell-permeable mTOR inhibitor. Med.Chem.Commun. 4 471

If you know of a relevant reference for eCF 309, please let us know.

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Citations for eCF 309

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
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Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

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Pain Research Product Guide

A collection of over 280 products for pain research, the guide includes research tools for the study of:

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Pathways for eCF 309


mTOR Signaling Pathway

mTOR is a serine/threonine kinase that nucleates at multiprotein complexes mTORC1 and mTORC2. Signaling by these complexes regulates cell growth, proliferation and survival.