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Description: Cationic agent for liposome and LNP formation
Alternative Names: 1,2-dioleoyl-3-trimethylammoniumpropane
Chemical Name: N,N,N-Trimethyl-2,3-bis(oleoyloxy)propan-1-aminium chloride
Purity: ≥95% (HPLC)
Protocols (1)

Biological Activity for DOTAP

DOTAP is a cationic agent used in liposome formation. Facilitates the transfection of DNA, RNA, and other molecules with negative charges into eukaryotic cells. Can be used to generate selective organ-targeting (SORT) lipid nanoparticles.

For more information on how to prepare MC3 lipid nanoparticles for RNA delivery, please see our protocol.

Technical Data for DOTAP

M. Wt 698.54
Formula C42H80ClNO4
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 132172-61-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DOTAP

Solvent Max Conc. mg/mL Max Conc. mM
ethanol 69.85 100
chloroform 69.85 100

Preparing Stock Solutions for DOTAP

The following data is based on the product molecular weight 698.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.43 mL 7.16 mL 14.32 mL
5 mM 0.29 mL 1.43 mL 2.86 mL
10 mM 0.14 mL 0.72 mL 1.43 mL
50 mM 0.03 mL 0.14 mL 0.29 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

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References for DOTAP

References are publications that support the biological activity of the product.

Wang et al (2023) Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery. Nat.Protoc. 18 265 PMID: 36316378

If you know of a relevant reference for DOTAP, please let us know.

Keywords: DOTAP, DOTAP supplier, LNP, lipid, nanoparticles, liposome, transfection, reagent, DNA, RNA, SORT, selective, organ, targeting, 1,2-dioleoyl-3-trimethylammoniumpropane, Lipid, Nanoparticles, Biochemicals, and, Molecular, Biology, 7179, Tocris Bioscience

Citations for DOTAP

Citations are publications that use Tocris products.

Currently there are no citations for DOTAP. Do you know of a great paper that uses DOTAP from Tocris? Please let us know.

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Protocols for DOTAP

The following protocol features additional information for the use of DOTAP (Cat. No. 7179).