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Caged calcium (Kd values are 48 and 19 nM at pH 7.2 and 7.4, respectively; Kd after uncaging = 2 mM). Selective over Mg2+ (Kd =10 mM). Rapid and efficient calcium release upon photolysis at 350 nm. Can also be used for two-photon uncaging. Extinction coefficient of 5120 M-1 cm-1, quantum yield 0.09. Cell permeable.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 849.74. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.18 mL||5.88 mL||11.77 mL|
|5 mM||0.24 mL||1.18 mL||2.35 mL|
|10 mM||0.12 mL||0.59 mL||1.18 mL|
|50 mM||0.02 mL||0.12 mL||0.24 mL|
References are publications that support the biological activity of the product.
Wang et al (2015) Spontaneous activity of cochlear hair cells triggered by fluid secretion mechanism in adjacent support cells Cell 163 1348 PMID: 26627734
Gordon et al (2008) Brain metabolism dictates the polarity of astrocyte control over arterioles. Nature 456 745 PMID: 18971930
Ellis-Davies et al (2006) Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties. Cell Calcium 39 75 PMID: 16303177
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