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Description: Fluorescent ROS indicator
Chemical Name: 5-Ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Dihydroethidium

Dihydroethidium is a fluorescent reactive oxygen species indicator. Cell permeable and suitable for use in live cells. Can be used with Mito-HE (Cat. No. 7641) to measure superoxide in living cells. Excitation/emission maxima λ 350/595 nm

Optical Data for Dihydroethidium

Dihydroethidium Dye Spectra
Emission Color Yellow
λabs 500 nm
λem 582 nm
Extinction Coefficient (ε) 14000 M-1cm-1
Cell Permeable Yes
Application Flow Cytometry, Confocal Imaging

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Technical Data for Dihydroethidium

M. Wt 315.41
Formula C21H21N3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 104821-25-2
PubChem ID 128682
Smiles NC=1C=CC=2C3=CC=C(N)C=C3C(C=4C=CC=CC4)N(C2C1)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Dihydroethidium

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.15 10

Preparing Stock Solutions for Dihydroethidium

The following data is based on the product molecular weight 315.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 31.7 mL 158.52 mL 317.05 mL
0.5 mM 6.34 mL 31.7 mL 63.41 mL
1 mM 3.17 mL 15.85 mL 31.7 mL
5 mM 0.63 mL 3.17 mL 6.34 mL

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Product Datasheets for Dihydroethidium

References for Dihydroethidium

References are publications that support the biological activity of the product.

Peterson et al (2002) Hydroethidine detection of superoxide production during the lithium-pilocarpine model of status epilepticus. Epilepsy Res. 49 226 PMID: 12076844

If you know of a relevant reference for Dihydroethidium, please let us know.

Keywords: Dihydroethidium, Dihydroethidium supplier, hydroethidium, ethidium, ROS, reactive, oxygen, species, superoxide, indicator, Fluorescent, Cell, Indicators, and, Sensors, 7642, Tocris Bioscience

Citations for Dihydroethidium

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals