Mito-HE

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Description: Fluorescent mitochondrial superoxide indicator in live cells
Chemical Name: [6-(3,8-Diamino-6-phenyl-5(6H)-phenanthridinyl)hexyl]triphenylphosphonium iodide
Purity: ≥80% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for Mito-HE

Mito-HE is a fluorescent probe for imaging superoxide in mitochondria of living cells. Selective for mitochondrial superoxides over other reactive oxygen and nitrogen species. Can be used to detect mitochondrial superoxide in Parkinson's and Alzheimer's disease in vitro models. Excitation/emission maxima λ ~ 510/580 nm.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Mito-HE

Emission Color Red
λabs 488 nm
λem 585 nm
Extinction Coefficient (ε) 11700 M-1cm-1
Cell Permeable Yes
Application Confocal imaging, Flow cytometery

Technical Data for Mito-HE

M. Wt 759.72
Formula C43H43IN3P
Storage Store at -20°C
Purity ≥80% (HPLC)
CAS Number 1003197-00-9
PubChem ID 160231797
InChI Key NMKOJFMSKHLXRV-UHFFFAOYSA-M
Smiles NC1=CC=C2C(C(C3=CC=CC=C3)N(C4=CC(N)=CC=C24)CCCCCC[P+](C5=CC=CC=C5)(C6=CC=CC=C6)C7=CC=CC=C7)=C1.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

References for Mito-HE

References are publications that support the biological activity of the product.

Rhee et al (2020) Modeling secondary iron overload cardiomyopathy with human induced pluripotent stem cell-derived cardiomyocytes. Cell Rep. 32 107886 PMID: 32668256

Renaudin et al (2021) BRCA2 deficiency reveals that oxidative stress impairs RNaseH1 function to cripple mitochondrial DNA maintenance. Cell Rep. 36 109478 PMID: 34348152

Mukhopadhyay et al (2007) Simple quantitative detection of mitochondrial superoxide production in live cells. Biochem.Biophys.Res.Commun. 358 203 PMID: 17475217

Mukhopadhyay et al (2007) Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy. Nat.Protoc. 2 2295 PMID: 17853886

Robinson et al (2006) Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc.Natl.Acad.Sci.U.S.A. 103 15038 PMID: 17015830

Chen et al (2020) Trilobatin protects against Aβ 25-35-induced hippocampal HT22 cells apoptosis through mediating ROS/p38/caspase 3-dependent pathway. Front.Pharmacol. 11 584 PMID: 32508629

Chung et al (2016) Parkin and PINK1 patient iPSC-derived midbrain dopamine neurons exhibit mitochondrial dysfunction and α-synuclein accumulation. Stem Cell Rep. 7 664 PMID: 27641647


If you know of a relevant reference for Mito-HE, please let us know.

Keywords: Mito-HE, Mito-HE supplier, MitoHE, red, mitochondrial, marker, stain, mitotracker, mitochondria, MitoSox, superoxide, indicator, Fluorescent, Cell, Indicators, and, Sensors, Other, Mitochondria, Dyes, Probes, 7641, Tocris Bioscience

Citations for Mito-HE

Citations are publications that use Tocris products.

Currently there are no citations for Mito-HE. Do you know of a great paper that uses Mito-HE from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals