Pricing Availability   Qty
Description: γ-secretase inhibitor; inhibits Notch pathway
Alternative Names: Dibenzazepine, YO-01027
Chemical Name: N-[(1S)-2-[[(7S)-6,7-Dihydro-5-methyl-6-oxo-5H-dibenz[b,d]azepin-7-yl]amino]-1-methyl-2-oxoethyl]-3,5-difluorobenzeneacetamide
Purity: ≥98% (HPLC)
Citations (11)
Reviews (1)
Literature (3)

Biological Activity for DBZ

DBZ is an inhibitor of γ-secretase aspartyl protease. DBZ blocks amyloid precursor protein-like (APPL) and Notch cleavage (IC50 values are 2.6 nM and 1.7-2.9 nM, respectively). By inhibiting Notch and the Notch pathway, DBZ improves efficiency of iPSC generation and production of iPSCs from mouse and human keratinocytes without KLF4 and CMYC. In an animal model of Alzheimer's disease, DBZ inhibits the cleavage of amyloid precursor protein into Aβ40. DBZ promotes cochlear supporting cell proliferation and hair cell mitotic regeneration in neonatal mice. DBZ in combination with iDOT1L enables maintenance of human naïve-state pluripotency in non-hypoxic conditions.

DBZ synthesized to Ancillary Material Grade also available.

Compound Libraries for DBZ

DBZ is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for DBZ

M. Wt 463.48
Formula C26H23F2N3O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 209984-56-5
PubChem ID 11454028
Smiles CN1C2=C(C=CC=C2)C3=C(C=CC=C3)[C@H](NC([C@@H](NC(CC4=CC(F)=CC(F)=C4)=O)C)=O)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DBZ

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 46.35 100

Preparing Stock Solutions for DBZ

The following data is based on the product molecular weight 463.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.16 mL 10.79 mL 21.58 mL
5 mM 0.43 mL 2.16 mL 4.32 mL
10 mM 0.22 mL 1.08 mL 2.16 mL
50 mM 0.04 mL 0.22 mL 0.43 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for DBZ

Certificate of Analysis / Product Datasheet
Select another batch:

References for DBZ

References are publications that support the biological activity of the product.

Groth et al (2010) Pharmacological analysis of Drosophila melanogaster γ-secretase with respect to differential proteolysis of Notch and APP. Mol.Pharmacol. 77 567 PMID: 20064975

van Es et al (2005) Notch/γ-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells. Nature 435 959 PMID: 15959515

Ichida et al (2014) Notch inhibition allows oncogene-independent generation of iPS cells. Nat.Chem.Biol. 10 632 PMID: 24952596

Wu et al (2020) Dibenzazepine promotes cochlear supporting cell proliferation and hair cell regeneration in neonatal mice. Cell Prolif. 53 e12872 PMID: 32677724

Isono et al (2020) The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human naïve-state pluripotency in non-hypoxic conditions. Regen.Ther. 15 161 PMID: 33426214

If you know of a relevant reference for DBZ, please let us know.

View Related Products by Product Action

View all γ-Secretase Inhibitors

Keywords: DBZ, DBZ supplier, Notch, inhibitors, inhibits, gamma, γ, g-secretases, signaling, signalling, pathways, gamma-secretase, amyloid, stem, cells, iPSCs, induced, pluripotent, reprogramming, dedifferentiation, YO01027, YO-01027, alzheimers, APP, Ab, Abeta, Dibenzazepine, Gamma-Secretase, Stem, Cell, Reprogramming, Organoids, 4489, Tocris Bioscience

11 Citations for DBZ

Citations are publications that use Tocris products. Selected citations for DBZ include:

Yung et al (2019) Sufu- And Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals. Nat.Commun. 10 4647 PMID: 31604927

Santos et al (2019) Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity. Nat.Commun. 10 4407 PMID: 31562298

Marshall et al (2018) Analysis of the Intrinsic Self-Organising Properties of Mesenchymal Stromal Cells in Three-Dimensional Co-Culture Models with Endothelial Cells. Bioengineering (Basel) 5 PMID: 30373192

Bi et al (2016) Stage-specific effects of Notch activation during skeletal myogenesis. Elife 5 PMID: 27644105

Do you know of a great paper that uses DBZ from Tocris? Please let us know.

Reviews for DBZ

Average Rating: 3 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used DBZ?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

By Minjin Jeong on 09/09/2019
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: iPSCs

Differentiation of hiPSCs into optic progenitor cells.

For obtaining a higher solubility, I recommend warming at 37°C.

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Research Product Guide

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products
Alzheimer's Disease Poster

Alzheimer's Disease Poster

Alzheimer's disease (AD) is a debilitating and progressive neurodegenerative disease and the most common cause of dementia, affecting approximately 30% of individuals aged over 85 years. This poster summarizes the cellular and molecular mechanisms of AD.