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Description: Fluorogenic Zn(II) sensor for isolation of stem cell derived β cells
Chemical Name: 3',6'-bis(Acetyloxy)-4',5'-bis[[bis(2-pyridinylmethyl)amino]methyl]-2',7'-dichlorospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for DA ZP1

DA ZP1 (diacetylated Zinpyr1) is a fluorogenic Zn(II) sensor (Kd Zn2+ = 0.6 nM). It can be used to image and isolate pancreatic β-cells and β-like cells in vitro. Used to purify live stem cell-derived β-like cells. It can image transplanted islet grafts and endogenous mouse islets in vivo. DA ZP1 is non-fluorescent in the absence of Zn(II) ions but its binding with Zn(II) promotes hydrolytic cleavage of the acetyl groups, generating a strong fluorescent signal. Excitation and emission maxima (λ) are 490 nm and 522 nm, respectively.

Optical Data for DA ZP1

λabs 490 nm
λem 522 nm
Cell Permeable Yes
Application Flow Cytometry

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Technical Data for DA ZP1

M. Wt 907.81
Formula C50H40Cl2N6O7
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 1816254-69-9
PubChem ID 86305621
Smiles CC(OC1=C(C=C2C(OC3=C(C(OC(C)=O)=C(C=C3C24OC(C5=CC=CC=C45)=O)Cl)CN(CC6=CC=CC=N6)CC7=CC=CC=N7)=C1CN(CC8=CC=CC=N8)CC9=CC=CC=N9)Cl)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DA ZP1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 1.82 2

Preparing Stock Solutions for DA ZP1

The following data is based on the product molecular weight 907.81. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.02 mM 55.08 mL 275.39 mL 550.78 mL
0.1 mM 11.02 mL 55.08 mL 110.16 mL
0.2 mM 5.51 mL 27.54 mL 55.08 mL
1 mM 1.1 mL 5.51 mL 11.02 mL

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References for DA ZP1

References are publications that support the biological activity of the product.

Kahraman et al (2021) Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cells. Life Sci.Alliance 4 e202000840 PMID: 33514654

Zastrow et al (2016) Reaction-based probes for imaging mobile zinc in live cells and tissues. ACS Sens. 32 32 PMID: 26878065

If you know of a relevant reference for DA ZP1, please let us know.

Keywords: DA ZP1, DA ZP1 supplier, DAZP1, DA-ZP1, fluorogenic, Zn, (II), sensor, fluorescence, fluorescent, probe, islet, β-cells, β-like, cells, indicators, sensors, ions, zinc, stem, cell-derived, beta, -like, purification, , in, vivo, Fluorescent, Cell, Indicators, and, Sensors, Ion, 7444, Tocris Bioscience

Citations for DA ZP1

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Fluorescent Dyes and Probes Research Product Guide

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