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Description: Near-infrared probe that detects Aβ40 aggregates. Used for: amyloid-β detection in vitro and in vivo. Application: fluorescence microscopy, epifluorescence microscopy
Chemical Name: (T-4)-[(1E,6E)-1,7-Bis[4-(dimethylamino)phenyl]-1,6-heptadiene-3,5-dionato-kO3,kO5]difluoroboron
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for CRANAD 2

Key information: CRANAD 2 is a curcumin derivatized near-infrared probe that binds to Aβ40 aggregates. Penetrates the blood-brain barrier.

Used for: amyloid- detection in vitro, tissue and in vivo. Shown to bind to plaques in APP-PS1 transgenic mice, in vitro. Detects senile plaques in 19-month-old Tg2576 mice in vivo.

Application: Fluorescence microscopy, epifluorescence microscopy.

Properties and Photophysical Data: CRANAD 2 binds to Aβ40 aggregates (Kd = 38 nM) and elicits upon interacting with Aβ aggregates, an emission blue shift (from 805 nm to 715 nm). When unbound in PBS, excitation and emission maxima (λ) are 640 nm and 805 nm, respectively.

Technical Data for CRANAD 2

M. Wt 410.26
Formula C23H25BF2N2O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1193447-34-5
PubChem ID 46861929
Smiles CN(C)C(C=C3)=CC=C3/C=C/C1=CC(/C=C/C2=CC=C(N(C)C)C=C2)=[O+][B-](F)(F)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for CRANAD 2

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 2.05 5 with gentle warming

Preparing Stock Solutions for CRANAD 2

The following data is based on the product molecular weight 410.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 48.75 mL 243.75 mL 487.5 mL
0.25 mM 9.75 mL 48.75 mL 97.5 mL
0.5 mM 4.87 mL 24.37 mL 48.75 mL
2.5 mM 0.97 mL 4.87 mL 9.75 mL

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References for CRANAD 2

References are publications that support the biological activity of the product.

Ran et al (2009) Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-beta deposits. J.Am.Chem.Soc. 131 15257 PMID: 19807070

Ran et al (2011) Non-conjugated small molecule FRET for differentiating monomers from higher molecular weight amyloid beta species. PLoS One. 6 e19362 PMID: 21559413

Ni et al (2021) In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice. Photoacoustics 23 100285 PMID: 34354924

If you know of a relevant reference for CRANAD 2, please let us know.

Keywords: CRANAD 2, CRANAD 2 supplier, near-infrared, NIR, probes, AB40, AB, amyloid-beta, beta-amyloid, aggregates, senile, plaques, Tg2576, amyloidb, amyloidβ, Amyloid, Beta, Peptides, Fluorescent, B, Probes, 4803, Tocris Bioscience

Citations for CRANAD 2

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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