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Submit ReviewChromaLIVE™ Non-Toxic Dye New
Description: Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells
Purity: ≥90% (HPLC)
Biological Activity for ChromaLIVE™ Non-Toxic Dye
ChromaLIVE™ Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content phenotypic screening of live cells. It allows live cell phenotypic imaging with a single dye enabling multi-organelle phenotypic readout to accurately differentiate cellular phenotypes stress and cell death pathways. Excitation and emission maxima (λ) are 488 nM and 561 nM, respectively. ChromaLIVE™ Non-Toxic Dye is provided as 10 μL of 1000x concentrate in DMSO.
Please refer to this protocol for further information on how to use this product.
ChromaLIVE™ is a registered trademark of Saguaro Biosciences.
Scientific Data
Live-cell Imaging
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ChromaLIVE™ Non-Toxic Dye Application. High-content imaging application of HeLa cells treated with staurosporine for 20 hours, then stained with ChromaLIVE™ Non-Toxic Dye and Hoechst 33342. Image acquired on the Yokogawa CQ1.
Live-cell Imaging
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ChromaLIVE™ Non-Toxic Dye Application. High-content imaging of live MCF-7 cells stained with ChromaLIVE™ Non-Toxic Dye, imaged at 561–600 nm and 488–700 nm. Demonstrates live-cell compatibility and bright cytoplasmic signal.
Live-cell Imaging
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ChromaLIVE™ Non-Toxic Dye Application High-content imaging of HEK293 spheroids stained with ChromaLIVE™ Non-Toxic Dye and imaged using the Yokogawa CQ3000. Six-tile panel displays treatment conditions left to right: DMSO, rapamycin (50 µM), and brefeldin A (10 µM); timepoints top to bottom: 24 h and 48 h.
Licensing Information
Sold under license from Saguaro Biosciences
Optical Data for ChromaLIVE™ Non-Toxic Dye
| λabs | 488 nm |
|---|---|
| λem | 561 nm |
| Cell Permeable | Yes |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for ChromaLIVE™ Non-Toxic Dye
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for ChromaLIVE™ Non-Toxic Dye
| Solubility | Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x) |
Product Datasheets for ChromaLIVE™ Non-Toxic Dye
References for ChromaLIVE™ Non-Toxic Dye
References are publications that support the biological activity of the product.
Sivagurunathan et al (2025) Alternate dyes for image-based profiling assays. SLAS Discov. PMID: 40885286
If you know of a relevant reference for ChromaLIVE™ Non-Toxic Dye, please let us know.
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Citations for ChromaLIVE™ Non-Toxic Dye
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents