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ChromaLIVE™ Deep Red Non-Toxic Dye

Cat. No. 9009 Submit a Review Datasheet / COA / SDS
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Description: Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells, can be multiplexed with NucleoLIVE
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Biological Activity Scientific Data Technical Data Solubility Calculators Datasheets References

Biological Activity

ChromaLIVE™ Deep Red Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content phenotypic screening of live cells. Optimised for live cell painting and morphological profiling in 2D monolayers, 3D spheroids or organoids, the dye provides multi organelle read out for unbiased classification of cell states such as proliferation, stress responses, autophagy, apoptosis or other phenotypic transitions. ChromaLIVE™ Deep Red Non-Toxic Dye can be used with NucleoLIVE™ Non-Toxic Dye (Cat. No. 8935) to get even more insight from live cell painting assays.

Please refer to this protocol for further information on how to use this product.

ChromaLIVE™ is a trademark of Saguaro Biosciences.

Scientific Data

Fluorescence Imaging ChromaLIVE™ Deep Red Non-Toxic Dye High-Content Imaging View Larger

ChromaLIVE™ Deep Red Non-Toxic Dye High-Content Imaging.  MCF-7 cells treated with 0.1% DMSO and stained with ChromaLIVE™ Deep Red Non-Toxic Dye and NucleoLIVE™ Non-Toxic Dye (Catalog # 8935) for multiplex phenotypic analysis.

Fluorescence Imaging ChromaLIVE™ Deep Red Non-Toxic Dye High-Content Imaging View Larger

ChromaLIVE™ Deep Red Non-Toxic Dye High-Content Imaging.  MCF-7 cells treated with 1 nM thapsigargin for 24 hours, analyzed through ChromaLIVE™ Deep Red Non-Toxic Dye and NucleoLIVE™ Non-Toxic Dye (Catalog # 8935) staining for stress response characterization.

Fluorescence Imaging ChromaLIVE™ Deep Red Non-Toxic Dye High-Content Imaging.  View Larger

ChromaLIVE™ Deep Red Non-Toxic Dye High-Content Imaging.  MCF-7 cells treated with 1 µM tunicamycin for 24 hours to induce ER stress, co-stained with ChromaLIVE™ Deep Red Non-Toxic Dye and NucleoLIVE™ Non-Toxic Dye (Catalog # 8935) for detailed phenotypic analysis.

Fluorescence Imaging Multiplexed Phenotypic Imaging with ChromaLIVE™ Deep Red and ER-LIVE™ Endoplasmic Reticulum Dye View Larger

Multiplexed Phenotypic Imaging. U2OS cells treated with DMSO, co-stained with ChromaLIVE™ Deep Red Non-Toxic Dye and ER-LIVE™ Endoplasmic Reticulum Dye (Catalog # 9010) for high-content analysis of subcellular structures.

Licensing Information

Sold under license from Saguaro Biosciences

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

Storage Store at -20°C
Purity ≥90% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solubility Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x)

References

References are publications that support the biological activity of the product.

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Citations for ChromaLIVE™ Deep Red Non-Toxic Dye

Citations are publications that use Tocris products.

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Protocols for ChromaLIVE™ Deep Red Non-Toxic Dye

The following protocol features additional information for the use of ChromaLIVE™ Deep Red Non-Toxic Dye (Cat. No. 9009).