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Biological Activity for Cholesterol
Cholesterol is a major sterol in all mammalian organisms; it is an essential component that makes up about 20-25% of the structural lipids of cell membranes. Cholesterol determines the permeability and fluidity characteristics of the cell membranes. Cholesterol is an endogenous ligand for estrogen-related receptor α (ERRα). It is also a precursor in several biosynthetic pathways, including steroid hormones and the active form of vitamin D. Cholesterol is a component of lipid nanoparticles (LNPs), and is important in modulating membrane stability, with the effect being context dependent. When combined with lipids that have low gel-liquid crystalline phase transition temperature (Tm), Cholesterol reduces membrane fluidity and increases bilayer thickness; when combined with high Tm lipids, it increases membrane fluidity and narrows the bilayer thickness.
For more information on how to prepare MC3 lipid nanoparticles for RNA delivery, please see our protocol.
Technical Data for Cholesterol
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Cholesterol
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|ethanol||19.33||50 with gentle warming|
Preparing Stock Solutions for Cholesterol
The following data is based on the product molecular weight 386.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.59 mL||12.93 mL||25.86 mL|
|5 mM||0.52 mL||2.59 mL||5.17 mL|
|10 mM||0.26 mL||1.29 mL||2.59 mL|
|50 mM||0.05 mL||0.26 mL||0.52 mL|
References for Cholesterol
References are publications that support the biological activity of the product.
Tenchov et al (2021) Lipid nanoparticles - from liposomes to mRNA vaccine delivery, a landscape of research diversity and advancement. ACS Nano 15 16982 PMID: 34181394
Cheng et al (2018) Dendrimer-based lipid nanoparticles deliver therapeutic fah mRNA to normalize liver function and extend survival in a mouse model of hepatorenal tyrosinemia type I. Adv.Mater 30 e1805308 PMID: 30368954
Ghanbari et al (2020) Cholesterol as an endogenous ligand of ERRα promotes ERRα-mediated cellular proliferation and metabolic target gene expression in breast cancer cells. Cell 9 1765 PMID: 32717915
If you know of a relevant reference for Cholesterol, please let us know.
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Keywords: Cholesterol, Cholesterol supplier, Cholesterol, structural, lipid, membrane, fluidity, nanoparticle, LNP, estrogen-related, receptor, alpha, ERRa, Biochemicals, and, Molecular, Biology, Estrogen, Related, Receptors, Lipid, Nanoparticles, 7945, Tocris Bioscience
Citations for Cholesterol
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