Calcein AM

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Cat.No. 5119 - Calcein AM | C46H46N2O23
Description: Cell permeable compound; hydrolyzed to become fluorescent in living cells
Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester
Purity: ≥95% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Cell permeable, non fluorescent compound; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells. Used for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.

Technical Data

M. Wt 994.86
Formula C46H46N2O23
Storage Store at -20°C
Purity ≥95% (HPLC)
PubChem ID 4126474
InChI Key XKFSBWQWNMZWFA-UHFFFAOYSA-N
Smiles O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 100 mM in DMSO

Preparing Stock Solutions

The following data is based on the product molecular weight 994.86. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.01 mL 5.03 mL 10.05 mL
5 mM 0.2 mL 1.01 mL 2.01 mL
10 mM 0.1 mL 0.5 mL 1.01 mL
50 mM 0.02 mL 0.1 mL 0.2 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322 PMID: 8647833

Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328 PMID: 9305892

Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391 PMID: 20980255

De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189 PMID: 8737602


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Keywords: Calcein AM, supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Fluorescent, Probes, Multidrug, Transporters, Fluorescent, Probes, Tocris Bioscience

Citations for Calcein AM

Citations are publications that use Tocris products.

Currently there are no citations for Calcein AM. Do you know of a great paper that uses Calcein AM from Tocris? If so please let us know.

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Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for Calcein AM

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