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Submit ReviewCalcein AM
Description: Cell permeable non-fluorescent compound; green-fluorescent in living cells once hydrolyzed. Used for: cell tracing and cell viability monitoring. Application: fluorescent microscopy and flow cytometry
Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester
Purity: ≥90% (HPLC)
Biological Activity for Calcein AM
Key information: Calcein AM is a cell permeable non-fluorescent compound, that becomes green fluorescent once hydrolyzed in live cells.
Used for: cell tracing, monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.
Application: fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: in live cells, non-fluorescent Calcein AM is hydrolyzed by intracellular esterases into green-fluorescent calcein, which is retained in the cytoplasm. Excitation and emission maxima (λ) are 495 nm and 515 nm, respectively.
It is recommended to prepare stock solutions of Calcein AM in DMSO.
Optical Data for Calcein AM
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 515 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescent Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Calcein AM
| M. Wt | 994.86 |
| Formula | C46H46N2O23 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 890090-35-4 |
| PubChem ID | 4126474 |
| InChI Key | XKFSBWQWNMZWFA-UHFFFAOYSA-N |
| Smiles | O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Calcein AM
References for Calcein AM
References are publications that support the biological activity of the product.
Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322 PMID: 8647833
Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328 PMID: 9305892
Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391 PMID: 20980255
De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189 PMID: 8737602
If you know of a relevant reference for Calcein AM, please let us know.
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Keywords: Calcein AM, Calcein AM supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Fluorescent, Cell, Indicators, and, Sensors, Multidrug, Transporters, Viability, Stains, Dyes, Flow, Cytometry, 5119, Tocris Bioscience
5 Citations for Calcein AM
Citations are publications that use Tocris products. Selected citations for Calcein AM include:
Walsh et al (2018) Loss of the mitochondrial kinase PINK1 does not alter platelet function. Sci Rep 8 14377 PMID: 30258205
Guo et al (2018) Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration. Stem Cell Res Ther 9 281 PMID: 30359318
Yannick D et al (2022) Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity. STAR Protoc 3 101218 PMID: 35265864
Jiahn-Chun et al (2020) Interleukin-1β Enhances Umbilical Cord Mesenchymal Stem Cell Adhesion Ability on Human Umbilical Vein Endothelial Cells via LFA-1/ICAM-1 Interaction. Stem Cells Int 2019 7267142 PMID: 31949440
Zhang et al (2018) CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression. Cell Death Dis 9 1101 PMID: 30368520
Do you know of a great paper that uses Calcein AM from Tocris? Please let us know.
Reviews for Calcein AM
Average Rating: 4.7 (Based on 3 Reviews.)
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MCF7 Spheroids viability.
By
Caterina Suelzu on 11/26/2024
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: MCF7
MCF7 cells spheroids were stained with 1 uM Calcein AM for 1.5h
HCT116 spheroids.
By
Gabriele Strusi on 11/26/2024
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HCT116
Viability staining of HCT116 spheroids 1 uM concentration
cell viability assay.
By
Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: motor neurons
For analyzing cellular toxicity in neurons
Neurons were treated with 50 uM glutamate for 1 hour. To assess the neuronal viability, neurons were incubated with calcein-AM (2 uM), and ethidium homodimer (4 uM) in HEPES buffer for 45-60 min at 37 degrees, rinsed with HEPES 4-5 times and examined under an epifluorescence microscope. This assay clearly distinguished live cells stained positive for calcein (green) from the dead cells stained positive for ethidium homodimer (red).
Literature in this Area
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents