Calcein AM

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Cat.No. 5119 - Calcein AM | C46H46N2O23
Description: Cell permeable compound; hydrolyzed to become fluorescent in living cells
Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester
Purity: ≥95% (HPLC)
Datasheet
Citations (3)
Reviews (1)
Literature (1)

Biological Activity for Calcein AM

Calcein AM is a cell permeable, non-fluorescent compound for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells..

Optical Data for Calcein AM

λabs 495 nm
λem 515 nm
Application Flow cytometry

Technical Data for Calcein AM

M. Wt 994.86
Formula C46H46N2O23
Storage Store at -20°C
Purity ≥95% (HPLC)
PubChem ID 4126474
InChI Key XKFSBWQWNMZWFA-UHFFFAOYSA-N
Smiles O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Calcein AM

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 100

Preparing Stock Solutions for Calcein AM

The following data is based on the product molecular weight 994.86. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.01 mL 5.03 mL 10.05 mL
5 mM 0.2 mL 1.01 mL 2.01 mL
10 mM 0.1 mL 0.5 mL 1.01 mL
50 mM 0.02 mL 0.1 mL 0.2 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References for Calcein AM

References are publications that support the biological activity of the product.

Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322 PMID: 8647833

Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328 PMID: 9305892

Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391 PMID: 20980255

De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189 PMID: 8737602


If you know of a relevant reference for Calcein AM, please let us know.

Keywords: Calcein AM, Calcein AM supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Fluorescent, Cell, Indicators, and, Sensors, Multidrug, Transporters, Viability, Stains, Dyes, Flow, Cytometry, 5119, Tocris Bioscience

3 Citations for Calcein AM

Citations are publications that use Tocris products. Selected citations for Calcein AM include:

Zhang et al (2018) CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression. Cell Death Dis 9 1101 PMID: 30368520

Guo et al (2018) Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration. Stem Cell Res Ther 9 281 PMID: 30359318

Walsh et al (2018) Loss of the mitochondrial kinase PINK1 does not alter platelet function. Sci Rep 8 14377 PMID: 30258205


Do you know of a great paper that uses Calcein AM from Tocris? Please let us know.

Reviews for Calcein AM

Average Rating: 4 (Based on 1 Review.)

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cell viability assay.
By Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: motor neurons

For analyzing cellular toxicity in neurons

Neurons were treated with 50 uM glutamate for 1 hour. To assess the neuronal viability, neurons were incubated with calcein-AM (2 uM), and ethidium homodimer (4 uM) in HEPES buffer for 45-60 min at 37 degrees, rinsed with HEPES 4-5 times and examined under an epifluorescence microscope. This assay clearly distinguished live cells stained positive for calcein (green) from the dead cells stained positive for ethidium homodimer (red).

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Literature in this Area

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*Please note that Tocris will only send literature to established scientific business / institute addresses.


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