Calcein AM
Biological Activity
Cell permeable, non fluorescent compound; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells. Used for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.
Technical Data
| M. Wt | 994.86 |
| Formula | C46H46N2O23 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| PubChem ID | 4126474 |
| InChI Key | XKFSBWQWNMZWFA-UHFFFAOYSA-N |
| Smiles | O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 100 mM in DMSO |
Preparing Stock Solutions
The following data is based on the product molecular weight 994.86. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.01 mL | 5.03 mL | 10.05 mL |
| 5 mM | 0.2 mL | 1.01 mL | 2.01 mL |
| 10 mM | 0.1 mL | 0.5 mL | 1.01 mL |
| 50 mM | 0.02 mL | 0.1 mL | 0.2 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322 PMID: 8647833
Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328 PMID: 9305892
Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391 PMID: 20980255
De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189 PMID: 8737602
If you know of a relevant reference for Calcein AM, please let us know.
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Keywords: Calcein AM, supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Fluorescent, Probes, Multidrug, Transporters, Fluorescent, Probes, Tocris Bioscience
Citations for Calcein AM
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