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Cell permeable, non-fluorescent compound for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells..
Optical Data for Calcein AM
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 994.86. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.01 mL||5.03 mL||10.05 mL|
|5 mM||0.2 mL||1.01 mL||2.01 mL|
|10 mM||0.1 mL||0.5 mL||1.01 mL|
|50 mM||0.02 mL||0.1 mL||0.2 mL|
References are publications that support the biological activity of the product.
Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322 PMID: 8647833
Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328 PMID: 9305892
Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391 PMID: 20980255
De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189 PMID: 8737602
If you know of a relevant reference for Calcein AM, please let us know.
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Keywords: Calcein AM, Calcein AM supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Fluorescent, Cell, Indicators, and, Sensors, Multidrug, Transporters, Viability, Stains, Dyes, Flow, Cytometry, 5119, Tocris Bioscience
3 Citations for Calcein AM
Citations are publications that use Tocris products. Selected citations for Calcein AM include:
Zhang et al (2018) CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression. Cell Death Dis 9 1101 PMID: 30368520
Guo et al (2018) Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration. Stem Cell Res Ther 9 281 PMID: 30359318
Walsh et al (2018) Loss of the mitochondrial kinase PINK1 does not alter platelet function. Sci Rep 8 14377 PMID: 30258205
Do you know of a great paper that uses Calcein AM from Tocris? Please let us know.
Reviews for Calcein AM
Average Rating: 4 (Based on 1 Review.)
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For analyzing cellular toxicity in neurons
Neurons were treated with 50 uM glutamate for 1 hour. To assess the neuronal viability, neurons were incubated with calcein-AM (2 uM), and ethidium homodimer (4 uM) in HEPES buffer for 45-60 min at 37 degrees, rinsed with HEPES 4-5 times and examined under an epifluorescence microscope. This assay clearly distinguished live cells stained positive for calcein (green) from the dead cells stained positive for ethidium homodimer (red).
Literature in this Area
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