Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewCalcein AM
Description: Cell permeable compound; hydrolyzed to become fluorescent in living cells
Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester
Purity: ≥95% (HPLC)
Biological Activity
Cell permeable, non-fluorescent compound for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells..
Technical Data
| M. Wt | 994.86 |
| Formula | C46H46N2O23 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| PubChem ID | 4126474 |
| InChI Key | XKFSBWQWNMZWFA-UHFFFAOYSA-N |
| Smiles | O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solubility | Soluble to 100 mM in DMSO |
Product Datasheets
References
References are publications that support the biological activity of the product.
Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322 PMID: 8647833
Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328 PMID: 9305892
Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391 PMID: 20980255
De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189 PMID: 8737602
If you know of a relevant reference for Calcein AM, please let us know.
View Related Products by Target
Keywords: Calcein AM, Calcein AM supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Fluorescent, Cell, Indicators, and, Sensors, Multidrug, Transporters, 5119, Tocris Bioscience
3 Citations for Calcein AM
Citations are publications that use Tocris products. Selected citations for Calcein AM include:
Walsh et al (2018) Loss of the mitochondrial kinase PINK1 does not alter platelet function. Sci Rep 8 14377 PMID: 30258205
Zhang et al (2018) CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression. Cell Death Dis 9 1101 PMID: 30368520
Guo et al (2018) Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration. Stem Cell Res Ther 9 281 PMID: 30359318
Do you know of a great paper that uses Calcein AM from Tocris? Please let us know.
Reviews for Calcein AM
Average Rating: 4 (Based on 1 Review.)
Have you used Calcein AM?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
cell viability assay.
By
Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: motor neurons
For analyzing cellular toxicity in neurons
Neurons were treated with 50 uM glutamate for 1 hour. To assess the neuronal viability, neurons were incubated with calcein-AM (2 uM), and ethidium homodimer (4 uM) in HEPES buffer for 45-60 min at 37 degrees, rinsed with HEPES 4-5 times and examined under an epifluorescence microscope. This assay clearly distinguished live cells stained positive for calcein (green) from the dead cells stained positive for ethidium homodimer (red).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide [Spring/Summer 2019]New
Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.
- 7-TM Receptors
- Enzymes
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Transporters
- Chemogenetics
- Epigenetics
- Fluorescent Imaging
- PROTACs
- Signal Transduction
- Stem Cells