Calcein AM
Biological Activity
Cell permeable, non-fluorescent compound for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells..
Technical Data
| M. Wt | 994.86 |
| Formula | C46H46N2O23 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| PubChem ID | 4126474 |
| InChI Key | XKFSBWQWNMZWFA-UHFFFAOYSA-N |
| Smiles | O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solubility | Soluble to 100 mM in DMSO |
Product Datasheets
References
References are publications that support the biological activity of the product.
Bakos et al (1996) Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 271 12322 PMID: 8647833
Lazarowski et al (1997) Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 272 24328 PMID: 9305892
Kuehn et al (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 286 391 PMID: 20980255
De Gendt et al (1996) The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 249 189 PMID: 8737602
If you know of a relevant reference for Calcein AM, please let us know.
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Keywords: Calcein AM, Calcein AM supplier, CAS, 148504-34-1, fluorescent, probes, agents, general, cells, viability, chemotaxis, adhesion, multidrug, resistance, MDR, Fluorescent, Cell, Indicators, and, Sensors, Multidrug, Transporters, 5119, Tocris Bioscience
1 Citation for Calcein AM
Citations are publications that use Tocris products. Selected citations for Calcein AM include:
Guo et al (2018) Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration. Stem Cell Res Ther 9 281 PMID: 30359318
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Reviews for Calcein AM
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For analyzing cellular toxicity in neurons
Neurons were treated with 50 uM glutamate for 1 hour. To assess the neuronal viability, neurons were incubated with calcein-AM (2 uM), and ethidium homodimer (4 uM) in HEPES buffer for 45-60 min at 37 degrees, rinsed with HEPES 4-5 times and examined under an epifluorescence microscope. This assay clearly distinguished live cells stained positive for calcein (green) from the dead cells stained positive for ethidium homodimer (red).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
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