BRD 0539

Pricing Availability   Qty
Description: Cell permeable and reversible Cas9 inhibitor
Chemical Name: (3aR,4R,9bR)-8-(2-Fluorophenyl)-2,3,3a,4,5,9b-hexahydro-1-[(4-methylphenyl)sulfonyl]-1H-pyrrolo[3,2-c]quinoline-4-methanol
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for BRD 0539

BRD 0539 is a cell-permeable and reversible SpCas9 (Streptococcus pyogenes Cas9) inhibitor. BRD 0539 shows dose-dependent SpCas9 inhibition in an in vitro DNA cleavage assay (apparent IC50 = 22 μM) and in an eGFP disruption assay (apparent EC50 = 11 μM). Allows dose and temporal control of SpCas9-based systems.

Licensing Information

Sold under licence from the Broad Institute

Technical Data for BRD 0539

M. Wt 452.54
Formula C25H25FN2O3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1403838-79-8
PubChem ID 54666326
Smiles [H][C@]12CCN([C@@]1([H])C1=CC(=CC=C1N[C@H]2CO)C1=C(F)C=CC=C1)[S](=O)(=O)C1=CC=C(C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BRD 0539

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 45.25 100
ethanol 45.25 100

Preparing Stock Solutions for BRD 0539

The following data is based on the product molecular weight 452.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.21 mL 11.05 mL 22.1 mL
5 mM 0.44 mL 2.21 mL 4.42 mL
10 mM 0.22 mL 1.1 mL 2.21 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

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References for BRD 0539

References are publications that support the biological activity of the product.

Maji et al (2019) A high-throughput platform to identify small-molecule inhibitors of CRISPR-Cas9. Cell 177 1067 PMID: 31051099

If you know of a relevant reference for BRD 0539, please let us know.

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Citations for BRD 0539

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