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Biological Activity for BRD 0539
BRD 0539 is a cell-permeable and reversible SpCas9 (Streptococcus pyogenes Cas9) inhibitor. BRD 0539 shows dose-dependent SpCas9 inhibition in an in vitro DNA cleavage assay (apparent IC50 = 22 μM) and in an eGFP disruption assay (apparent EC50 = 11 μM). Allows dose and temporal control of SpCas9-based systems.
Sold under licence from the Broad Institute
Technical Data for BRD 0539
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BRD 0539
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for BRD 0539
The following data is based on the product molecular weight 452.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.21 mL||11.05 mL||22.1 mL|
|5 mM||0.44 mL||2.21 mL||4.42 mL|
|10 mM||0.22 mL||1.1 mL||2.21 mL|
|50 mM||0.04 mL||0.22 mL||0.44 mL|
References for BRD 0539
References are publications that support the biological activity of the product.
Maji et al (2019) A high-throughput platform to identify small-molecule inhibitors of CRISPR-Cas9. Cell 177 1067 PMID: 31051099
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Citations for BRD 0539
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Literature in this Area
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