BRD 0539

Pricing Availability   Qty
Cat.No. 7153 - BRD 0539 | C25H25FN2O3S | CAS No. 1403838-79-8
Description: Cell permeable and reversible Cas9 inhibitor
Chemical Name: (3aR,4R,9bR)-8-(2-Fluorophenyl)-2,3,3a,4,5,9b-hexahydro-1-[(4-methylphenyl)sulfonyl]-1H-pyrrolo[3,2-c]quinoline-4-methanol
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for BRD 0539

BRD 0539 is a cell-permeable and reversible SpCas9 (Streptococcus pyogenes Cas9) inhibitor. BRD 0539 shows dose-dependent SpCas9 inhibition in an in vitro DNA cleavage assay (apparent IC50 = 22 μM) and in an eGFP disruption assay (apparent EC50 = 11 μM). Allows dose and temporal control of SpCas9-based systems.

Licensing Information

Sold under licence from the Broad Institute

Technical Data for BRD 0539

M. Wt 452.54
Formula C25H25FN2O3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1403838-79-8
PubChem ID 54666326
InChI Key CZOIXFMISSBIJL-DCEDVJGZSA-N
Smiles [H][C@]12CCN([C@@]1([H])C1=CC(=CC=C1N[C@H]2CO)C1=C(F)C=CC=C1)[S](=O)(=O)C1=CC=C(C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BRD 0539

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 45.25 100
ethanol 45.25 100

Preparing Stock Solutions for BRD 0539

The following data is based on the product molecular weight 452.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.21 mL 11.05 mL 22.1 mL
5 mM 0.44 mL 2.21 mL 4.42 mL
10 mM 0.22 mL 1.1 mL 2.21 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for BRD 0539

References are publications that support the biological activity of the product.

Maji et al (2019) A high-throughput platform to identify small-molecule inhibitors of CRISPR-Cas9. Cell 177 1067 PMID: 31051099


If you know of a relevant reference for BRD 0539, please let us know.

View Related Products by Target

Keywords: BRD 0539, BRD 0539 supplier, BRD0539, inhibits, inhibitors, Cas9, SpCas9, reversible, specific, CRISPR, Reagents, 7153, Tocris Bioscience

Citations for BRD 0539

Citations are publications that use Tocris products.

Currently there are no citations for BRD 0539. Do you know of a great paper that uses BRD 0539 from Tocris? Please let us know.

Reviews for BRD 0539

There are currently no reviews for this product. Be the first to review BRD 0539 and earn rewards!

Have you used BRD 0539?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Stem Cell

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products