Biocytin

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Cat.No. 3349 - Biocytin | C16H28N4O4S | CAS No. 576-19-2
Description: Versatile marker for neuroanatomical investigations
Chemical Name: N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine
Datasheet
Citations (7)
Literature

Biological Activity

Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Technical Data

M. Wt 372.48
Formula C16H28N4O4S
Storage Store at -20°C
CAS Number 576-19-2
PubChem ID 83814
InChI Key BAQMYDQNMFBZNA-MNXVOIDGSA-N
Smiles O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@H](N)C(O)=O)=O)([H])N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1.1eq. NaOH 37.25 100
water 18.62 50mM with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 372.48. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.68 mL 13.42 mL 26.85 mL
5 mM 0.54 mL 2.68 mL 5.37 mL
10 mM 0.27 mL 1.34 mL 2.68 mL
50 mM 0.05 mL 0.27 mL 0.54 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Kobbert et al (2000) Current concepts in neuroanatomical tracing. Prog.Neurobiol. 62 327 PMID: 10856608

Roth et al (2004) Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 478 35 PMID: 15334648

MacNeil and Gaul (2008) Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 506 6 PMID: 17990268


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Keywords: Biocytin, supplier, Versatile, marker, neuroanatomical, investigations, reagents, anterograde, retrograde, Biochemicals, and, Molecular, Biology, Biochemicals, and, Molecular, Biology, Tocris Bioscience

7 Citations for Biocytin

Citations are publications that use Tocris products. Selected citations for Biocytin include:

Tsai et al (2012) Autistic-like behaviour and cerebellar dysfunction in Purkinje cell Tsc1 mutant mice. MBio 488 647 PMID: 22763451

Jerome et al (2011) Parallel optical control of spatiotemporal neuronal spike activity using high-speed digital light processing. Front Syst Neurosci 5 70 PMID: 21904526

Lam et al (2011) Leptin does not directly affect CNS serotonin neurons to influence appetite. Acta Crystallogr F Struct Biol Commun 13 584 PMID: 21531340

Robinson and Wyatt (2015) Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase. Nature 71 586 PMID: 25945713

Dettmann et al (2014) Isolation, expansion and transplantation of postnatal murine progenitor cells of the enteric nervous system. PLoS One 9 e97792 PMID: 24871092

Xia et al (2014) Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons. PLoS One 9 e100968 PMID: 24971996

Cunningham et al (2014) hPSC-derived maturing GABAergic interneurons ameliorate seizures and abnormal behavior in epileptic mice. Cell Stem Cell 15 559 PMID: 25517465


Do you know of a great paper that uses Biocytin from Tocris? If so please let us know.

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