Biocytin

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Cat.No. 3349 - Biocytin | C16H28N4O4S | CAS No. 576-19-2
Description: Versatile marker for neuroanatomical investigations
Chemical Name: N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine
Datasheet
Citations (12)
Reviews

Biological Activity for Biocytin

Biocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Technical Data for Biocytin

M. Wt 372.48
Formula C16H28N4O4S
Storage Store at -20°C
CAS Number 576-19-2
PubChem ID 83814
InChI Key BAQMYDQNMFBZNA-MNXVOIDGSA-N
Smiles O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@H](N)C(O)=O)=O)([H])N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Biocytin

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 18.62 50 with gentle warming

Preparing Stock Solutions for Biocytin

The following data is based on the product molecular weight 372.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 5.37 mL 26.85 mL 53.69 mL
2.5 mM 1.07 mL 5.37 mL 10.74 mL
5 mM 0.54 mL 2.68 mL 5.37 mL
25 mM 0.11 mL 0.54 mL 1.07 mL

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Product Datasheets for Biocytin

Certificate of Analysis / Product Datasheet
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References for Biocytin

References are publications that support the biological activity of the product.

Kobbert et al (2000) Current concepts in neuroanatomical tracing. Prog.Neurobiol. 62 327 PMID: 10856608

Roth et al (2004) Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 478 35 PMID: 15334648

MacNeil and Gaul (2008) Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 506 6 PMID: 17990268


If you know of a relevant reference for Biocytin, please let us know.

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Keywords: Biocytin, Biocytin supplier, Versatile, marker, neuroanatomical, investigations, reagents, anterograde, retrograde, Biochemicals, and, Molecular, Biology, 3349, Tocris Bioscience

12 Citations for Biocytin

Citations are publications that use Tocris products. Selected citations for Biocytin include:

Roesler et al (2019) Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Front Cell Neurosci 13 330 PMID: 31474830

Robinson and Wyatt (2015) Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase. Nature 71 586 PMID: 25945713

Jerome et al (2011) Parallel optical control of spatiotemporal neuronal spike activity using high-speed digital light processing. Front Syst Neurosci 5 70 PMID: 21904526

Ferrarese et al (2018) Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. Cell Rep 24 3455 PMID: 30257207


Do you know of a great paper that uses Biocytin from Tocris? Please let us know.

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