Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|water||18.62||50mM with gentle warming|
Preparing Stock Solutions
The following data is based on the product molecular weight 372.48. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.68 mL||13.42 mL||26.85 mL|
|5 mM||0.54 mL||2.68 mL||5.37 mL|
|10 mM||0.27 mL||1.34 mL||2.68 mL|
|50 mM||0.05 mL||0.27 mL||0.54 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Kobbert et al (2000) Current concepts in neuroanatomical tracing. Prog.Neurobiol. 62 327 PMID: 10856608
Roth et al (2004) Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 478 35 PMID: 15334648
MacNeil and Gaul (2008) Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 506 6 PMID: 17990268
If you know of a relevant reference for Biocytin, please let us know.
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Keywords: Biocytin, supplier, Versatile, marker, neuroanatomical, investigations, reagents, anterograde, retrograde, Biochemicals, and, Molecular, Biology, Tocris Bioscience
7 Citations for Biocytin
Citations are publications that use Tocris products. Selected citations for Biocytin include:
Tsai et al (2012) Autistic-like behaviour and cerebellar dysfunction in Purkinje cell Tsc1 mutant mice. MBio 488 647 PMID: 22763451
Jerome et al (2011) Parallel optical control of spatiotemporal neuronal spike activity using high-speed digital light processing. Front Syst Neurosci 5 70 PMID: 21904526
Lam et al (2011) Leptin does not directly affect CNS serotonin neurons to influence appetite. Acta Crystallogr F Struct Biol Commun 13 584 PMID: 21531340
Robinson and Wyatt (2015) Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase. Nature 71 586 PMID: 25945713
Dettmann et al (2014) Isolation, expansion and transplantation of postnatal murine progenitor cells of the enteric nervous system. PLoS One 9 e97792 PMID: 24871092
Xia et al (2014) Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons. PLoS One 9 e100968 PMID: 24971996
Cunningham et al (2014) hPSC-derived maturing GABAergic interneurons ameliorate seizures and abnormal behavior in epileptic mice. Cell Stem Cell 15 559 PMID: 25517465
Do you know of a great paper that uses Biocytin from Tocris? If so please let us know.
Literature in this Area
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