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Chemical dimerizer used in auxin-inducible degron (AID) systems. Induces degradation of a target protein tagged with the Tir1 E3 ligase AID in human colorectral cancer and mouse ES cells. Endogenous plant hormone.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 175.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.71 mL||28.54 mL||57.08 mL|
|5 mM||1.14 mL||5.71 mL||11.42 mL|
|10 mM||0.57 mL||2.85 mL||5.71 mL|
|50 mM||0.11 mL||0.57 mL||1.14 mL|
References are publications that support the biological activity of the product.
Nishimura et al (2009) An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat.Methods 6 917 PMID: 19915560
Natsume et al (2016) Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 210 PMID: 27052166
If you know of a relevant reference for Auxin, please let us know.
Keywords: Auxin, Auxin supplier, Auxin-inducible, degron, AID, targeted, protein, degradation, TPD, chemical, dimerizer, TIR1, phytohormone, Protein, Dimerizers, Biochemicals, and, Molecular, Biology, 6834, Tocris Bioscience
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