Pricing Availability   Qty
Description: Building block for the lysine acylation using conjugating enzymes (LACE) technique
Chemical Name: (2R)-2-(Acetylamino)-3-mercapto-N-methylpropanamide
Literature (1)

Biological Activity for Ac-Cys-NHMe

Ac-Cys-NHMe is a non-canonical amino acid that is an essential building block for lysine acylation using conjugating enzymes (LACE) technique. LACE is a chemoenzymatic approach that uses an E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, Ubc9, to conjugate peptide thioesters containing a C-terminal ubiquitin-derived sequence (LRLRGG) to a lysine residue located in a recognition sequence (IKQE) on a protein via an isopeptide bond. Ac-Cys-NHMe can be reacted with a peptide hydrazide to form a reactive peptide thioester, which is the substrate for Ubc9. LACE enables the site-specific modification of proteins at lysine residues to allow attachment of biochemical probes such as fluorophores to proteins or to engineer proteins for site-specific delivery.

Technical Data for Ac-Cys-NHMe

M. Wt 176.23
Formula C6H12N2O2S
Storage Store at -20°C
CAS Number 10061-65-1
PubChem ID 11949287
Smiles CNC([C@H](CS)NC(C)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Ac-Cys-NHMe

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 17.62 100
water 17.62 100

Preparing Stock Solutions for Ac-Cys-NHMe

The following data is based on the product molecular weight 176.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.67 mL 28.37 mL 56.74 mL
5 mM 1.13 mL 5.67 mL 11.35 mL
10 mM 0.57 mL 2.84 mL 5.67 mL
50 mM 0.11 mL 0.57 mL 1.13 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Ac-Cys-NHMe

Certificate of Analysis / Product Datasheet
Select another batch:

References for Ac-Cys-NHMe

References are publications that support the biological activity of the product.

Levasseur et al (2022) Post-assembly modification of protein cages by Ubc9-mediated lysine acylation. Chembiochem 23 e202200332 PMID: 35951442

Hofmann et al (2020) Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins. Nat.Chem. 12 1008 PMID: 32929246

If you know of a relevant reference for Ac-Cys-NHMe, please let us know.

Keywords: Ac-Cys-NHMe, Ac-Cys-NHMe supplier, lysine, acylation, using, conjugating, enzymes, LACE, SUMO, conjugation, Ubc9, Unnatural, Amino, Acids, Biochemicals, and, Molecular, Biology, 7936, Tocris Bioscience

Citations for Ac-Cys-NHMe

Citations are publications that use Tocris products.

Currently there are no citations for Ac-Cys-NHMe. Do you know of a great paper that uses Ac-Cys-NHMe from Tocris? Please let us know.

Reviews for Ac-Cys-NHMe

There are currently no reviews for this product. Be the first to review Ac-Cys-NHMe and earn rewards!

Have you used Ac-Cys-NHMe?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals