4-Thiouridine

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Cat.No. 7005 - 4-Thiouridine | C9H12N2O5S | CAS No. 13957-31-8
Description: Photoreactive uridine analog for RNA sequencing
Chemical Name: 4-Thiouridine
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Photoreactive uridine analog for RNA sequencing (RNA seq) and thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq). Used as a crosslinker to identify RNA binding proteins. Is incorporated into newly transcribed RNA and can be used to track RNA from synthesis to degradation.

Technical Data

M. Wt 260.26
Formula C9H12N2O5S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 13957-31-8
PubChem ID 3032615
InChI Key ZLOIGESWDJYCTF-XVFCMESISA-N
Smiles OC[C@H]1O[C@@H](N2C=CC(NC2=O)=S)[C@@H]([C@@H]1O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 26.03 100
DMSO 26.03 100

Preparing Stock Solutions

The following data is based on the product molecular weight 260.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.84 mL 19.21 mL 38.42 mL
5 mM 0.77 mL 3.84 mL 7.68 mL
10 mM 0.38 mL 1.92 mL 3.84 mL
50 mM 0.08 mL 0.38 mL 0.77 mL

Molarity Calculator

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References

References are publications that support the biological activity of the product.

Garibaldi et al (2017) Isolation of newly transcribed RNA using the metabolic label 4-thiouridine. Methods Mol.Biol. 1648 169 PMID: 28766297

Herzog et al (2017) Thiol-linked alkylation of RNA to assess expression dynamics. Nat.Methods 14 1198 PMID: 28945705

Schofield et al (2018) TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding. Nat.Methods 15 221 PMID: 29355846


If you know of a relevant reference for 4-Thiouridine, please let us know.

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Citations for 4-Thiouridine

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