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Description: Photoreactive uridine analog for RNA sequencing
Chemical Name: 4-Thiouridine
Purity: ≥98% (HPLC)
Citations (2)

Biological Activity for 4-Thiouridine

4-Thiouridine is a photoreactive uridine analog for RNA sequencing (RNA seq) and thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq). Used as a crosslinker to identify RNA binding proteins. Is incorporated into newly transcribed RNA and can be used to track RNA from synthesis to degradation.

Technical Data for 4-Thiouridine

M. Wt 260.26
Formula C9H12N2O5S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 13957-31-8
PubChem ID 3032615
Smiles OC[C@H]1O[C@@H](N2C=CC(NC2=O)=S)[C@@H]([C@@H]1O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 4-Thiouridine

Solvent Max Conc. mg/mL Max Conc. mM
water 26.03 100
DMSO 26.03 100

Preparing Stock Solutions for 4-Thiouridine

The following data is based on the product molecular weight 260.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.84 mL 19.21 mL 38.42 mL
5 mM 0.77 mL 3.84 mL 7.68 mL
10 mM 0.38 mL 1.92 mL 3.84 mL
50 mM 0.08 mL 0.38 mL 0.77 mL

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Product Datasheets for 4-Thiouridine

References for 4-Thiouridine

References are publications that support the biological activity of the product.

Garibaldi et al (2017) Isolation of newly transcribed RNA using the metabolic label 4-thiouridine. Methods Mol.Biol. 1648 169 PMID: 28766297

Herzog et al (2017) Thiol-linked alkylation of RNA to assess expression dynamics. Nat.Methods 14 1198 PMID: 28945705

Schofield et al (2018) TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding. Nat.Methods 15 221 PMID: 29355846

If you know of a relevant reference for 4-Thiouridine, please let us know.

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Keywords: 4-Thiouridine, 4-Thiouridine supplier, 4sU, tagging, photoreactive, labels, labelling, labeling, RNA, seq, sequencing, SLAM, TimeLapse, Biochemicals, and, Molecular, Biology, 7005, Tocris Bioscience

2 Citations for 4-Thiouridine

Citations are publications that use Tocris products. Selected citations for 4-Thiouridine include:

Cindy et al (2023) In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion. Nat Commun 14 3386 PMID: 37296170

Ali et al (2021) Genetic Deletion of Polo-Like Kinase 2 Induces a Pro-Fibrotic Pulmonary Phenotype. Cells 10 PMID: 33799608

Do you know of a great paper that uses 4-Thiouridine from Tocris? Please let us know.

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