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Photoreactive uridine analog for RNA sequencing (RNA seq) and thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq). Used as a crosslinker to identify RNA binding proteins. Is incorporated into newly transcribed RNA and can be used to track RNA from synthesis to degradation.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 260.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.84 mL||19.21 mL||38.42 mL|
|5 mM||0.77 mL||3.84 mL||7.68 mL|
|10 mM||0.38 mL||1.92 mL||3.84 mL|
|50 mM||0.08 mL||0.38 mL||0.77 mL|
References are publications that support the biological activity of the product.
Garibaldi et al (2017) Isolation of newly transcribed RNA using the metabolic label 4-thiouridine. Methods Mol.Biol. 1648 169 PMID: 28766297
Herzog et al (2017) Thiol-linked alkylation of RNA to assess expression dynamics. Nat.Methods 14 1198 PMID: 28945705
Schofield et al (2018) TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding. Nat.Methods 15 221 PMID: 29355846
If you know of a relevant reference for 4-Thiouridine, please let us know.
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Keywords: 4-Thiouridine, 4-Thiouridine supplier, 4sU, tagging, photoreactive, labels, labelling, labeling, RNA, seq, sequencing, SLAM, TimeLapse, Biochemicals, and, Molecular, Biology, 7005, Tocris Bioscience
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