3-Cyano-7-ethoxycoumarin

Pricing Availability Delivery Time Qty
Cat.No. 4815 - 3-Cyano-7-ethoxycoumarin | C12H9NO3 | CAS No. 117620-77-6
Description: Fluorescent cytochrome P450 substrate
Chemical Name: 7-Ethoxy-2-oxo-2H-1-benzopyran-3-carbonitrile
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Fluorescent P450 substrate (excitation/emission wavelengths = 408/455 nm); metabolized to cyano-hydroxycoumarin.

Technical Data

M. Wt 215.2
Formula C12H9NO3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 117620-77-6
PubChem ID 164045
InChI Key YAFGHMIAFYQSCF-UHFFFAOYSA-N
Smiles O=C2C(C#N)=CC1=CC=C(OCC)C=C1O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 50 mM in DMSO

Preparing Stock Solutions

The following data is based on the product molecular weight 215.2. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.65 mL 23.23 mL 46.47 mL
5 mM 0.93 mL 4.65 mL 9.29 mL
10 mM 0.46 mL 2.32 mL 4.65 mL
50 mM 0.09 mL 0.46 mL 0.93 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

White et al (1988) A continuous fluorometric assay for cytochrome P-450-dependent mixed function oxidases using 3-cyano-7-ethoxycoumarin. Anal.Biochem. 172 304 PMID: 3189781

Donato et al (2004) Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab.Dispos. 32 699 PMID: 15205384


If you know of a relevant reference for 3-Cyano-7-ethoxycoumarin, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Cytochrome P450 Substrates

Keywords: 3-Cyano-7-ethoxycoumarin, supplier, CEC, Cytochrome, P450, CYP450, CYP2A1, substrates, fluorescent, probes, Cytochrome, P450, Tocris Bioscience

Citations for 3-Cyano-7-ethoxycoumarin

Citations are publications that use Tocris products.

Currently there are no citations for 3-Cyano-7-ethoxycoumarin. Do you know of a great paper that uses 3-Cyano-7-ethoxycoumarin from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for 3-Cyano-7-ethoxycoumarin

Protocols

TODO: Add Protocols