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2B-(SP)

Cat. No. 1802 COA / Product Datasheet SDS
Pricing Availability Delivery Time Qty
Cat.No. 1802 - 2B-(SP) | Arg-Arg-Ala-Ala-Glu-Glu-Leu-Asp-Ser-Arg-Ala-Gly-pSer-Pro-Gln-Leu | CAS No. 186901-17-7
Description: Selective GSK-3 phosphopeptide substrate
Datasheet
Citations
Literature
Biological Activity Technical Data Solubility Calculators Datasheets

Biological Activity

Selective phosphopeptide substrate for glycogen synthase kinase-3 (GSK-3); derived from the phosphorylation site of the translation factor eIF2B.

Technical Data

M. Wt 1835.88
Formula C71H123N26O29P
Sequence RRAAEELDSRAGSPQL

(Modifications: Ser-13 = pSer)
Storage Desiccate at -20°C
CAS Number 186901-17-7
PubChem ID 90488714
InChI Key LBVCQYQHTKHZRY-APZQELAWSA-N
Smiles [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](COP(O)(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 1 mg/ml in water

Preparing Stock Solutions

The following data is based on the product molecular weight 1835.88. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.54 mL 2.72 mL 5.45 mL
5 mM 0.11 mL 0.54 mL 1.09 mL
10 mM 0.05 mL 0.27 mL 0.54 mL
50 mM 0.01 mL 0.05 mL 0.11 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets

Certificate of Analysis / Product Datasheet
Safety Datasheet

References

References are publications that support the products' biological activity.

Welsh et al (1997) Peptide substrates suitable for assaying glycogen synthase kinase-3 in crude cell extracts. Anal.Biochem. 244 16 PMID: 9025901

Williams et al (1999) Phosphorylated seryl and threonyl, but not tyrosyl, residues are efficient specificity determinants for GSK-3β and Shaggy. FEBS Lett. 448 86 PMID: 10217415

If you know of a relevant reference for 2B-(SP), please let us know.

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Keywords: Selective GSK-3 phosphopeptide Glycogen Synthase Kinases 3 Carbohydrate Metabolism Enzyme Substrates activators 2BSP Enzyme Substrates / Activators

Citations for 2B-(SP)

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