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2-Phospho-L-ascorbic acid trisodium salt
Stable ascorbic acid derivative used in cell culture; in combination with FGF-2, maintains differentiation potential in bone marrow-derived mesenchymal stem cells (MSC) through increased expression of HGF. Also exhibits synergistic protection of hMSCs under oxidative stress in combination with N-acetylcysteine (Cat. No. 5619).
|Storage||Desiccate at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 322.05. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.11 mL||15.53 mL||31.05 mL|
|5 mM||0.62 mL||3.11 mL||6.21 mL|
|10 mM||0.31 mL||1.55 mL||3.11 mL|
|50 mM||0.06 mL||0.31 mL||0.62 mL|
References are publications that support the biological activity of the product.
Falcon et al (2014) An in vitro cord formation assay identifies unique vascular phenotypes associated with angiogenic growth factors. PLoS One 9 e106901 PMID: 25210890
Bae et al (2015) L-ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. Growth Factors 33 71 PMID: 25714612
Li et al (2015) Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis. Sci.Rep. 5 9819 PMID: 25909282
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Citations for 2-Phospho-L-ascorbic acid trisodium salt
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.