N-Acetylcysteine amide

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Description: Glutathione (GSH) precursor; neuroprotective
Chemical Name: (2R)-2-(Acetylamino)-3-mercaptopropanamide
Citations (5)
Reviews (1)
Protocols (1)

Biological Activity for N-Acetylcysteine amide

N-Acetylcysteine amide is a glutathione (GSH) precursor and cell-permeable antioxidant. N-Acetylcysteine amide replenishes intracellular Glutathione (GSH). N-Acetylcysteine amide directly reduces intracellular Glutathione disulfide (GSSG) to GSH without glutathione peroxidase. N-Acetylcysteine amide has anti-inflammatory activity through regulation of activation of NF-κB and HIF-1α, as well as modulation of reactive oxygen species. N-Acetylcysteine amide improves neuronal mitochondrial bioenergetics, reduces tissue damage and enhances functional recovery following spinal cord injury in rats. N-Acetylcysteine amide also enhances behavioral recovery in rats following traumatic brain injury. Neuroprotective.

For more information about how N-Acetylcysteine amide may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Technical Data for N-Acetylcysteine amide

M. Wt 162.21
Formula C5H10N2O2S
Storage Store at -20°C
CAS Number 38520-57-9
PubChem ID 10176265
Smiles SC[C@H](NC(C)=O)C(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for N-Acetylcysteine amide

Solvent Max Conc. mg/mL Max Conc. mM
water 16.22 100
DMSO 16.22 100

Preparing Stock Solutions for N-Acetylcysteine amide

The following data is based on the product molecular weight 162.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 6.16 mL 30.82 mL 61.65 mL
5 mM 1.23 mL 6.16 mL 12.33 mL
10 mM 0.62 mL 3.08 mL 6.16 mL
50 mM 0.12 mL 0.62 mL 1.23 mL

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Product Datasheets for N-Acetylcysteine amide

Certificate of Analysis / Product Datasheet
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References for N-Acetylcysteine amide

References are publications that support the biological activity of the product.

Patel et al (2014) N-acetylcysteine amide preserves mitochondrial bioenergetics and improves functional recovery following spinal trauma. Exp.Neurol. 257 95 PMID: 24805071

Pandya et al (2014) N-acetylcysteine amide confers neuroprotection, improves bioenergetics and behavioral outcome following TBI. Exp.Neurol. 257 106 PMID: 24792639

Tu et al (2019) The anti-inflammatory and anti-oxidant mechanisms of the Keap1/Nrf2/ARE signaling pathway in chronic diseases. Aging Dis 10 637 PMID: 31165007

If you know of a relevant reference for N-Acetylcysteine amide, please let us know.

Keywords: N-Acetylcysteine amide, N-Acetylcysteine amide supplier, cell, permeable, antioxidant, neuroprotective, glutathione, precursor, GSH, organoids, hematopoietic, haematopoietic, stem, cells, HSC, Antioxidants, Hematopoietic, Stem, Cells, 5619, Tocris Bioscience

5 Citations for N-Acetylcysteine amide

Citations are publications that use Tocris products. Selected citations for N-Acetylcysteine amide include:

Tao et al (2021) Microengineered multi-organoid system from hiPSCs to recapitulate human liver-islet axis in normal and type 2 diabetes. Adv.Sci. 9 e2103495 PMID: 34951149

Craig et al (2022) Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia. Acta Neuropathol Commun 10 40 PMID: 35346366

Christian et al (2021) NF-κB determines Paneth versus goblet cell fate decision in the small intestine. Development 148 PMID: 34751748

Young-Min et al (2020) Nanosphere Loaded with Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus. Cells 9 PMID: 32151068

Do you know of a great paper that uses N-Acetylcysteine amide from Tocris? Please let us know.

Reviews for N-Acetylcysteine amide

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NAC inhibits HOCl-LDL-induced CaMKII oxidation.
By Chintan Koyani on 10/22/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Cardiomyocytes

HL-1 cardiomyocytes were pre-treated with Pyrrolidinedithiocarbamate (PDTC, 1 mM), Tempol (1 mM) or N-Acetylcysteine (NAC, 1 mM) for 30 min and stimulated with HOCl-LDL (200 µg/ml) for 2 h to follow CaMKII oxidation using Western blot.

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Protocols for N-Acetylcysteine amide

The following protocol features additional information for the use of N-Acetylcysteine amide (Cat. No. 5619).