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Description: Epithelial KCa channel activator
Chemical Name: 1-Ethyl-2-benzimidazolinone
Purity: ≥99% (HPLC)
Citations (16)
Reviews (1)

Biological Activity for 1-EBIO

1-EBIO is an activator of epithelial KCa channels, stimulates a large and sustained trans-epithelial Cl- secretory response across T84 monolayers. Induces hyperpolarization to the same magnitude as ACh in aortic value endothelial cells. Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes.

Compound Libraries for 1-EBIO

1-EBIO is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for 1-EBIO

M. Wt 162.19
Formula C9H10N2O
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 10045-45-1
PubChem ID 82320
Smiles CCN1C(=O)NC2=C1C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 1-EBIO

Solvent Max Conc. mg/mL Max Conc. mM
ethanol 16.22 100
DMSO 16.22 100

Preparing Stock Solutions for 1-EBIO

The following data is based on the product molecular weight 162.19. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 6.17 mL 30.83 mL 61.66 mL
5 mM 1.23 mL 6.17 mL 12.33 mL
10 mM 0.62 mL 3.08 mL 6.17 mL
50 mM 0.12 mL 0.62 mL 1.23 mL

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Product Datasheets for 1-EBIO

Certificate of Analysis / Product Datasheet
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References for 1-EBIO

References are publications that support the biological activity of the product.

Ayotunde and Adeagbo (1999) 1-Ethyl-2-benzimidazolinone stimulates endothelial KCa channels and nitric oxide formation in rat mesenteric vessels. Eur.J.Pharmacol. 379 151 PMID: 10497901

Devor et al (1996) Modulation of Cl- secretion by benzimidazolones. I. Direct activation of a Ca2+ - dependent K+ channel. Am.J.Physiol. 271 L775 PMID: 8944721

Kusama et al (2005) Reduced hyperpolarisation in endothelial cells of rabbit aortic value following chronic nitroglycerine administration. Br.J.Pharmacol. 146 487 PMID: 16056232

Kleger et al (2010) Modulation of calcium-activated potassium channels induces cardiogenesis of pluripotent stem cells and enrichment of pacemaker-like cells. Circulation. 122 1823 PMID: 20956206

If you know of a relevant reference for 1-EBIO, please let us know.

View Related Products by Product Action

View all Calcium-Activated Potassium (KCa) Channel Activators

Keywords: 1-EBIO, 1-EBIO supplier, Activator, epithelial, Kca, channels, Potassium, Channels, ca2+-activated, ca2+-dependent, K+, Ca2+-Activated, Cardiomyocyte, Stem, Cells, ESCs, and, iPSC, 1041, Tocris Bioscience

16 Citations for 1-EBIO

Citations are publications that use Tocris products. Selected citations for 1-EBIO include:

Benton et al (2013) Iberiotoxin-sensitive and -insensitive BK currents in Purkinje neuron somata. J Neurophysiol 109 2528 PMID: 23446695

Cuppoletti et al (2013) Methadone but not mor. inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents. Cell Biochem Biophys 66 53 PMID: 22918821

Lai et al (2011) PRL-3 promotes the proliferation of LoVo cells via the upregulation of KCNN4 channels. Oncol Rep 26 909 PMID: 21725609

Park et al (2011) Globotriaosylceramide leads to K(Ca)3.1 channel dysfunction: a new insight into endothelial dysfunction in Fabry disease. Cardiovasc Res 89 290 PMID: 20971723

Haddock et al (2011) Diet-induced obesity impairs endothelium-derived hyperpolarization via altered potassium channel signaling mechanisms. PLoS One 6 e16423 PMID: 21283658

Manaves et al (2004) Calcium and Vitamin D increase mRNA levels for the growth control hIK1 channel in human epidermal keratinocytes but functional channels are not observed. Korean J Physiol Pharmacol 4 7 PMID: 15200683

Banderali et al (2004) New insights on the voltage dependence of the KCa3.1 channel block by internal TBA. J Gen Physiol 124 333 PMID: 15452196

Gamble-George et al (2016) Cyclooxygenase-2 inhibition reduces stress-induced affective pathology. Elife 5 PMID: 27162170

Rau et al (2015) Increased Basolateral Amygdala Pyramidal Cell Excitability May Contribute to the Anxiogenic Phenotype Induced by Chronic Early-Life Stress. BMC Dermatol 35 9730 PMID: 26134655

Kusama et al (2005) Reduced hyperpolarization in endothelial cells of rabbit aortic valve following chronic nitroglycerine administration. Br J Pharmacol 146 487 PMID: 16056232

Wells et al (2016) Thalamic reticular impairment underlies attention deficit in Ptchd1(Y/-) mice. Nature 532 58 PMID: 27007844

Cuppoletti et al (2014) Differentiation between human ClC-2 and CFTR Cl- channels with pharmacological agents. Am J Physiol Cell Physiol 307 C479 PMID: 25009109

Duffy et al (2007) Adenosine closes the K+ channel KCa3.1 in human lung mast cells and inhibits their migration via the adenosine A2A receptor. Proc Natl Acad Sci U S A 37 1653 PMID: 17474152

Choi et al (2013) Contradictory Effects of Superoxide and Hydrogen Peroxide on KCa3.1 in Human Endothelial Cells. Eur J Immunol 17 181 PMID: 23776393

Cruse et al (2011) Functional KCa3.1 K+ channels are required for human fibrocyte migration. J Allergy Clin Immunol 128 1303 PMID: 21872912

Bushart et al (2018) Targeting potassium channels to treat cerebellar ataxia. Ann Clin Transl Neurol 5 297 PMID: 29560375

Do you know of a great paper that uses 1-EBIO from Tocris? Please let us know.

Reviews for 1-EBIO

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Study on BK channel activity.
By Eleanor Baxter on 10/23/2018
Assay Type: In Vitro
Species: Human

It activates Ca2+ activated K+ channels on epithelium. Our University lab uses it to study Big Conductance Channel channel opening.

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