1-EBIO

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Cat.No. 1041 - 1-EBIO | C9H10N2O | CAS No. 10045-45-1
Description: Activator of epithelial KCa channels
Chemical Name: 1-Ethyl-2-benzimidazolinone
Purity: ≥99% (HPLC)
Datasheet
Citations (7)
Literature

Biological Activity

Activator of epithelial KCa channels, stimulates a large and sustained trans-epithelial Cl- secretory response across T84 monolayers. Induces hyperpolarization to the same magnitude as ACh in aortic value endothelial cells. Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes.

Compound Libraries

1-EBIO is also offered as part of the Tocriscreen Plus and Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 162.19
Formula C9H10N2O
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 10045-45-1
PubChem ID 82320
InChI Key CXUCKELNYMZTRT-UHFFFAOYSA-N
Smiles CCN1C(=O)NC2=C1C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 16.22 100
ethanol 16.22 100
tris buffer solution 5.35 33mM with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 162.19. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 6.17 mL 30.83 mL 61.66 mL
5 mM 1.23 mL 6.17 mL 12.33 mL
10 mM 0.62 mL 3.08 mL 6.17 mL
50 mM 0.12 mL 0.62 mL 1.23 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Ayotunde and Adeagbo (1999) 1-Ethyl-2-benzimidazolinone stimulates endothelial KCa channels and nitric oxide formation in rat mesenteric vessels. Eur.J.Pharmacol. 379 151 PMID: 10497901

Devor et al (1996) Modulation of Cl- secretion by benzimidazolones. I. Direct activation of a Ca2+ - dependent K+ channel. Am.J.Physiol. 271 L775 PMID: 8944721

Kusama et al (2005) Reduced hyperpolarisation in endothelial cells of rabbit aortic value following chronic nitroglycerine administration. Br.J.Pharmacol. 146 487 PMID: 16056232

Kleger et al (2010) Modulation of calcium-activated potassium channels induces cardiogenesis of pluripotent stem cells and enrichment of pacemaker-like cells. Circulation. 122 1823 PMID: 20956206


If you know of a relevant reference for 1-EBIO, please let us know.

View Related Products by Product Action

View all Calcium-Activated Potassium (KCa) Channel Activators

Keywords: Activator epithelial Kca channels Potassium Channels ca2+-activated ca2+-dependent K+ Ca2+-Activated Potassium Channels

7 Citations for 1-EBIO

Citations are publications that use Tocris products. Selected citations for 1-EBIO include:

Rau et al (2015) Increased Basolateral Amygdala Pyramidal Cell Excitability May Contribute to the Anxiogenic Phenotype Induced by Chronic Early-Life Stress. BMC Dermatol 35 9730 PMID: 26134655

Benton et al (2013) Iberiotoxin-sensitive and -insensitive BK currents in Purkinje neuron somata. J Neurophysiol 109 2528 PMID: 23446695

Cuppoletti et al (2013) Methadone but not morphine inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents. Cell Biochem Biophys 66 53 PMID: 22918821

Choi et al (2013) Contradictory Effects of Superoxide and Hydrogen Peroxide on KCa3.1 in Human Endothelial Cells. Eur J Immunol 17 181 PMID: 23776393

Cruse et al (2011) Functional KCa3.1 K+ channels are required for human fibrocyte migration. J Allergy Clin Immunol 128 1303 PMID: 21872912

Duffy et al (2007) Adenosine closes the K+ channel KCa3.1 in human lung mast cells and inhibits their migration via the adenosine A2A receptor. Proc Natl Acad Sci U S A 37 1653 PMID: 17474152

Manaves et al (2004) Calcium and Vitamin D increase mRNA levels for the growth control hIK1 channel in human epidermal keratinocytes but functional channels are not observed. Korean J Physiol Pharmacol 4 7 PMID: 15200683


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