Z-DEVD-FMK

Pricing Availability Delivery Time Qty
Cat.No. 2166 - Z-DEVD-FMK | Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone
Description: Cell-permeable, irreversible caspase-3 inhibitor
Chemical Name: Benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone
Datasheet
Citations (4)
Literature

Biological Activity

Cell-permeable, irreversible inhibitor of caspase-3/CPP32; inhibits tumor cell apoptosis. Neuroprotective in rat hippocampus following seizures in vivo.

Technical Data

M. Wt 668.67
Formula C30H41FN4O12
Sequence DEVD

(Modifications: Asp-1 = Z-Asp(OMe), Glu-2 = Glu(OMe), Asp-4 = (OMe)-fluoromethylketone)

Storage Store at -20°C
PubChem ID 16760394
InChI Key GBJVAVGBSGRRKN-JYEBCORGSA-N
Smiles [H]N([C@@H](CC(=O)OC)C(=O)N[C@@H](CCC(=O)OC)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(=O)OC)C(=O)CF)C(=O)OCC1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 13.37 mg/ml in DMSO

Preparing Stock Solutions

The following data is based on the product molecular weight 668.67. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.5 mL 7.48 mL 14.96 mL
5 mM 0.3 mL 1.5 mL 2.99 mL
10 mM 0.15 mL 0.75 mL 1.5 mL
50 mM 0.03 mL 0.15 mL 0.3 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the products' biological activity.

Brocksted et al (1998) Identification of apoptosis-associated proteins in a human Burkitt lymphoma cell line. J.Biol.Chem. 273 28057 PMID: 9774422

Henshall et al (2000) Involvement of caspase-3-like protease in the mechanism of cell death following focally evoked limbic seizures. J.Neurochem. 74 1215 PMID: 10693954

Kugawa et al (2000) Apoptosis of NG108-15 cells induced by buprenorphine hydrochloride occurs via the caspase-3 pathway. Biol.Pharm.Bull. 23 930 PMID: 10963298


If you know of a relevant reference for Z-DEVD-FMK, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Caspase Inhibitors

Keywords: Cell-permeable irreversible caspase-3 inhibitors inhibits Caspases Proteinases Proteases Caspases

4 Citations for Z-DEVD-FMK

Citations are publications that use Tocris products. Selected citations for Z-DEVD-FMK include:

Wu et al (2016) Human genome-wide RNAi screen reveals host factors required for enterovirus 71 replication Nature Communications 7 13150 PMID: 27748395

Bill et al (2015) ANO1 interacts with EGFR and correlates with sensitivity to EGFR-targeting therapy in head and neck cancer. BMB Rep 6 9173 PMID: 25823819

Im-aram et al (2013) The mTORC2 component rictor contributes to cisplatin resistance in human ovarian cancer cells. PLoS One 8 e75455 PMID: 24086535

Woo et al (2012) Calpain-mediated processing of p53-associated parkin-like cytoplasmic protein (PARC) affects chemosensitivity of human ovarian cancer cells by promoting p53 subcellular trafficking. J Biol Chem 287 3963 PMID: 22117079


Do you know of a great paper that uses Z-DEVD-FMK from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Neurodegeneration

Neurodegeneration Product Guide

A collection of over 275 products for neurodegeneration research, the guide includes research tools for the study of:

  • Alzheimer's disease
  • Parkinson's disease
  • Huntington's disease
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Huntington's Disease

Huntington's Disease Poster

Huntington's disease (HD) is a monogenic neurodegenerative disorder, which is characterized by the prevalent loss of GABAergic medium spiny neurons (MSN) in the striatum. This poster summarizes the MSN intracellular signaling pathways implicated in the pathology of HD, as well as highlighting the use of iPSCs for HD modeling.

Parkinson's

Parkinson's Poster

Parkinson's disease (PD) causes chronic disability and is the second most common neurodegenerative condition. This poster outlines the neurobiology of the disease, as well as highlighting current therapeutic treatments for symptomatic PD, and emerging therapeutic strategies to delay PD onset and progression.

Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Z-DEVD-FMK

Protocols

TODO: Add Protocols