WS 12

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Cat.No. 3040 - WS 12 | C18H27NO2 | CAS No. 68489-09-8
Description: TRPM8 agonist; cooling agent
Chemical Name: (1R*,2S*)-N-(4-Methoxyphenyl)-5-methyl-2-(1-methylethyl)cyclohexanecarboxamide
Purity: ≥99% (HPLC)
Citations (1)
Reviews (1)

Biological Activity

Potent TRPM8 agonist that acts as a cooling agent (EC50 = 193 nM).

Technical Data

M. Wt 289.41
Formula C18H27NO2
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 68489-09-8
PubChem ID 11266244
Smiles C[C@H](C)[C@H]1[C@H]([C@@](NC2=CC=C(OC)C=C2)=O)C[C@H](C)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
ethanol 21.71 75

Preparing Stock Solutions

The following data is based on the product molecular weight 289.41. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.46 mL 17.28 mL 34.55 mL
5 mM 0.69 mL 3.46 mL 6.91 mL
10 mM 0.35 mL 1.73 mL 3.46 mL
50 mM 0.07 mL 0.35 mL 0.69 mL

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Product Datasheets

Safety Datasheet


References are publications that support the products' biological activity.

Beck et al (2007) Prospects for prostate cancer imaging and therapy using high-affinity TRPM8 activators. Cell Calcium 41 285 PMID: 16949669

Bodding et al (2007) Characterisation of TRPM8 as a pharmacophore receptor. Cell Calcium 42 618 PMID: 17517434

If you know of a relevant reference for WS 12, please let us know.

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Keywords: WS 12, supplier, TRPM8, agonists, cooling, agent, Channels, Transient, Receptor, Potential, WS12, TRPM, TRPM, Tocris Bioscience

1 Citation for WS 12

Citations are publications that use Tocris products. Selected citations for WS 12 include:

Morgan et al (2015) Genetic variants affecting human TRPA1 or TRPM8 structure can be classified in vitro as 'well expressed', 'poorly expressed' or 'salvageable'. Biosci Rep 35 PMID: 26330615

Do you know of a great paper that uses WS 12 from Tocris? If so please let us know.

Reviews for WS 12

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WS12 as selective TRPM8 agonist and better than menthol.
By Hiteshkumar Soni on 01/10/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Mouse Mesenteric artery

TRP Ion channel, TRPM8 agonist in in vivo experiments in mouse and in vitro tissue, especially Mouse Mesenteric artery to check change in arterial diameter.

review image

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