WS 12

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Description: TRPM8 agonist; cooling agent
Chemical Name: (1R*,2S*)-N-(4-Methoxyphenyl)-5-methyl-2-(1-methylethyl)cyclohexanecarboxamide
Purity: ≥99% (HPLC)
Citations (2)
Reviews (2)

Biological Activity for WS 12

WS 12 is a potent TRPM8 agonist that acts as a cooling agent (EC50 = 193 nM).

Technical Data for WS 12

M. Wt 289.41
Formula C18H27NO2
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 68489-09-8
PubChem ID 11266244
Smiles C[C@H](C)[C@H]1[C@H]([C@@](NC2=CC=C(OC)C=C2)=O)C[C@H](C)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for WS 12

Solvent Max Conc. mg/mL Max Conc. mM
ethanol 21.71 75

Preparing Stock Solutions for WS 12

The following data is based on the product molecular weight 289.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 4.61 mL 23.04 mL 46.07 mL
3.75 mM 0.92 mL 4.61 mL 9.21 mL
7.5 mM 0.46 mL 2.3 mL 4.61 mL
37.5 mM 0.09 mL 0.46 mL 0.92 mL

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Product Datasheets for WS 12

Certificate of Analysis / Product Datasheet
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References for WS 12

References are publications that support the biological activity of the product.

Beck et al (2007) Prospects for prostate cancer imaging and therapy using high-affinity TRPM8 activators. Cell Calcium 41 285 PMID: 16949669

Bodding et al (2007) Characterisation of TRPM8 as a pharmacophore receptor. Cell Calcium 42 618 PMID: 17517434

If you know of a relevant reference for WS 12, please let us know.

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Keywords: WS 12, WS 12 supplier, TRPM8, agonists, cooling, agent, Channels, Transient, Receptor, Potential, WS12, TRPM, 3040, Tocris Bioscience

2 Citations for WS 12

Citations are publications that use Tocris products. Selected citations for WS 12 include:

Morgan et al (2015) Genetic variants affecting human TRPA1 or TRPM8 structure can be classified in vitro as 'well expressed', 'poorly expressed' or 'salvageable'. Biosci Rep 35 PMID: 26330615

Liu et al (2013) TRPM8 is the principal mediator of menthol-induced analgesia of acute and inflammatory pain. Pain 154 2169 PMID: 23820004

Do you know of a great paper that uses WS 12 from Tocris? Please let us know.

Reviews for WS 12

Average Rating: 5 (Based on 2 Reviews.)

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Activation of TRPM8 currents.
By Anonymous on 01/18/2018
Assay Type: In Vitro
Species: Rat

WS12 was used to activate TRPM8 currents in dorsal root ganglia neurons through whole cell mode patch clamp.

WS12 as selective TRPM8 agonist and better than menthol..
By Hiteshkumar Soni on 01/10/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Mouse Mesenteric artery

TRP Ion channel, TRPM8 agonist in in vivo experiments in mouse and in vitro tissue, especially Mouse Mesenteric artery to check change in arterial diameter.

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