Withaferin A

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Cat.No. 2816 - Withaferin A | C28H38O6 | CAS No. 5119-48-2
Description: Inhibits NF-κB activation
Chemical Name: (4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
Purity: ≥98% (HPLC)
Datasheet
Citations (5)
Reviews (1)
Literature
Pathways

Biological Activity

Steroid lactone that displays anti-inflammatory, antitumor and antiangiogenic activity. Inhibits endothelial cells (HUVEC) sprouting in vitro (IC50 = 12 nM) and in vivo. Prevents NF-κB activation by inhibiting activation of IKKβ. Also inhibits chymotrypsin-like activity of the 20S proteasome.

Technical Data

M. Wt 470.6
Formula C28H38O6
Storage Desiccate at -20°C
Purity ≥98% (HPLC)
CAS Number 5119-48-2
PubChem ID 45489105
InChI Key DBRXOUCRJQVYJQ-WDBPBROTSA-N
Smiles O=C1[C@@]2(C)[C@@]([C@H]5C[C@]3([H])C2CC[C@@]4(C)[C@]([H])3CC[C@@H]4[C@@H]([C@]6([H])OC(C(CO)=C(C)C6)=O)C)(O5)[C@@H](O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.71 10
ethanol 2.35 5

Preparing Stock Solutions

The following data is based on the product molecular weight 470.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 21.25 mL 106.25 mL 212.49 mL
0.5 mM 4.25 mL 21.25 mL 42.5 mL
1 mM 2.12 mL 10.62 mL 21.25 mL
5 mM 0.42 mL 2.12 mL 4.25 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Mohan et al (2004) Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 7 115 PMID: 15516832

Yang et al (2007) The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from "Indian Winter Cherry". Mol.Pharmacol. 71 426 PMID: 17093135

Kaileh et al (2007) Withaferin A strongly elicits IkB kinase β hyperphosphorylation concomitant with potent inhibition of its kinase activity. J.Biol.Chem. 282 4253 PMID: 17150968


If you know of a relevant reference for Withaferin A, please let us know.

View Related Products by Product Action

View all NF-κB and IκB Inhibitors

Keywords: Withaferin A, Withaferin A supplier, inhibitors, inhibits, NF-κB, NF-kappaB, NF-kB, activation, Nuclear, Factor, Kappa, B, Cytokine, Signaling, Signalling, Transcription, Factors, WithaferinA, NF-kB/IkB, 2816, Tocris Bioscience

5 Citations for Withaferin A

Citations are publications that use Tocris products. Selected citations for Withaferin A include:

Yang et al (2016) The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages. Neural Plast 7 11385 PMID: 27150562

Martorana et al (2015) Withaferin A Inhibits Nuclear Factor-κB-Dependent Pro-Inflammatory and Stress Response Pathways in the Astrocytes. EMBO J 2015 381964 PMID: 26266054

Hatzold et al (2016) Tumor suppression in basal keratinocytes via dual non-cell-autonomous functions of a Na,K-ATPase beta subunit. Elife 5 PMID: 27240166

Trendelenburg et al (2012) TAK-1/p38/nNFκB signaling inhibits myoblast differentiation by increasing levels of AVNA. Skelet Muscle 2 3 PMID: 22313861

Yadav et al (2010) Celastrol suppresses invasion of colon and pancreatic cancer cells through the downregulation of expression of CXCR4 chemokine receptor. J Mol Med (Berl) 88 1243 PMID: 20798912


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Reviews for Withaferin A

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Withaferin inhibits LPS-induced TNFalpha production in macrophages.
By Christopher Trummer on 10/22/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Macrophages

RAW macrophages were stimulated with Withaferin (1 µM) alone or in the presence of LPS (200 ng/ml) for 8 h to follow TNFalpha mRNA expression using qPCR. Withaferin significantly inhibited LPS-induced TNFalpha production.

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Literature in this Area

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Pathways for Withaferin A

NF-κB

NF-κB Signaling Pathway

NF-κB signaling plays an important role in inflammation, the innate and adaptive immune response and stress. Dysregulated signaling can occur in inflammatory and autoimmune diseases.