Withaferin A

Pricing Availability   Qty
Description: Inhibits NF-κB activation
Chemical Name: (4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
Purity: ≥98% (HPLC)
Citations (7)
Reviews (2)
Pathways (1)

Biological Activity for Withaferin A

Withaferin A is a steroid lactone that displays anti-inflammatory, antitumor and antiangiogenic activity. Withaferin A inhibits endothelial cells (HUVEC) sprouting in vitro (IC50 = 12 nM) and in vivo, prevents NF-κB activation by inhibiting activation of IKKβ and inhibits chymotrypsin-like activity of the 20S proteasome. In an animal model of Huntington's disease (HD), Withaferin A delays disease progression and increases lifespan; in a cellular model it suppresses aggregation of mutant huntingtin, activates HSF1 and induces Hsp70 chaperone expression.

Technical Data for Withaferin A

M. Wt 470.6
Formula C28H38O6
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 5119-48-2
PubChem ID 45489105
Smiles O=C1[C@@]2(C)[C@@]([C@H]5C[C@]3([H])C2CC[C@@]4(C)[C@]([H])3CC[C@@H]4[C@@H]([C@]6([H])OC(C(CO)=C(C)C6)=O)C)(O5)[C@@H](O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Withaferin A

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 4.71 10

Preparing Stock Solutions for Withaferin A

The following data is based on the product molecular weight 470.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 21.25 mL 106.25 mL 212.49 mL
0.5 mM 4.25 mL 21.25 mL 42.5 mL
1 mM 2.12 mL 10.62 mL 21.25 mL
5 mM 0.42 mL 2.12 mL 4.25 mL

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Product Datasheets for Withaferin A

Certificate of Analysis / Product Datasheet
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References for Withaferin A

References are publications that support the biological activity of the product.

Mohan et al (2004) Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 7 115 PMID: 15516832

Yang et al (2007) The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from "Indian Winter Cherry". Mol.Pharmacol. 71 426 PMID: 17093135

Kaileh et al (2007) Withaferin A strongly elicits IkB kinase β hyperphosphorylation concomitant with potent inhibition of its kinase activity. J.Biol.Chem. 282 4253 PMID: 17150968

Joshi et al (2021) Withaferin A induces heat shock response and ameliorates disease progression in a mouse model of Huntington's disease. Mol.Neurobiol. 58 3992 PMID: 33904021

If you know of a relevant reference for Withaferin A, please let us know.

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7 Citations for Withaferin A

Citations are publications that use Tocris products. Selected citations for Withaferin A include:

Yang et al (2016) The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages. Neural Plast 7 11385 PMID: 27150562

Trendelenburg et al (2012) TAK-1/p38/nNFκB signaling inhibits myoblast differentiation by increasing levels of AVNA. Skelet Muscle 2 3 PMID: 22313861

Andersson et al (2018) Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis. JCI Insight 3 PMID: 30333314

Ryals et al (2018) The Inner Ear Heat Shock Transcriptional Signature Identifies Compounds That Protect Against Aminoglycoside Ototoxicity. Front Cell Neurosci 12 445 PMID: 30532693

Do you know of a great paper that uses Withaferin A from Tocris? Please let us know.

Reviews for Withaferin A

Average Rating: 4.5 (Based on 2 Reviews.)

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42 perturbagens used the zebrafish DASPEI screen.
By Anonymous on 12/17/2019
Assay Type: In Vivo
Species: Other
Cell Line/Tissue: larvae

Zebrafish larvae were placed into 48-well plates and used the DASPEI screen by 42 perturbagens drugs, including this compound

PMID: 30532693
review image

Withaferin inhibits LPS-induced TNFalpha production in macrophages.
By Christopher Trummer on 10/22/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Macrophages

RAW macrophages were stimulated with Withaferin (1 µM) alone or in the presence of LPS (200 ng/ml) for 8 h to follow TNFalpha mRNA expression using qPCR. Withaferin significantly inhibited LPS-induced TNFalpha production.

review image

Pathways for Withaferin A

NF-κB Signaling Pathway

NF-κB Signaling Pathway

NF-κB signaling plays an important role in inflammation, the innate and adaptive immune response and stress. Dysregulated signaling can occur in inflammatory and autoimmune diseases.