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Submit ReviewJanelia Fluor® 646 Tre New
Description: Red fluorescent trehalose probe for live mycobacteria imaging
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((((2R,3S,4S,5R,6R)-3,4,5-trihydroxy-6-(((2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)methyl)carbamoyl)benzoate
Purity: ≥95%
Biological Activity for Janelia Fluor® 646 Tre
Key information: Janelia Fluor® 646 Tre is a red fluorescent probe for imaging Mycobacterium tuberculosis, after metabolic incorporation of the trehalose component in the lipid-rich cell envelope.
Used for: imaging Mycobacterium tuberculosis in TB drug susceptibility testing.
Application: fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646 Tre
| Emission Color | Red |
|---|---|
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Correction Factor 280 | 0.19 |
| Application | Bacterial imaging |
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Technical Data for Janelia Fluor® 646 Tre
| M. Wt | 819.94 |
| Formula | C41H49N3O13Si |
| Storage | Store at -20°C |
| Purity | ≥95% |
| InChI Key | BZQLCLILAFFJOH-KEVSEYCPSA-N |
| Smiles | O[C@H]1[C@@H]([C@H](O[C@@H]([C@@H]1O)O[C@@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CNC(C3=CC=C(C([O-])=O)C(C(C(C=CC(N4CCC4)=C5)=C5[Si]6(C)C)=C7C6=C/C(C=C7)=[N+]8CCC\8)=C3)=O)O)O)O)CO)O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 646 Tre
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 8.2 | 10 |
Preparing Stock Solutions for Janelia Fluor® 646 Tre
The following data is based on the product molecular weight 819.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 12.2 mL | 60.98 mL | 121.96 mL |
| 0.5 mM | 2.44 mL | 12.2 mL | 24.39 mL |
| 1 mM | 1.22 mL | 6.1 mL | 12.2 mL |
| 5 mM | 0.24 mL | 1.22 mL | 2.44 mL |
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Product Datasheets for Janelia Fluor® 646 Tre
References for Janelia Fluor® 646 Tre
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Fluorescent Dyes and Probes Research Product GuideNew
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- ClariTSATM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals