TRIS base

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Cat.No. 3163 - TRIS base | C4H11NO3 | CAS No. 77-86-1
Description: Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
Chemical Name: 2-Amino-2-(hydroxymethyl)-1,3-propanediol
Purity: ≥99.5%
Datasheet
Citations
Reviews (2)

Biological Activity for TRIS base

TRIS base is a commonly used laboratory reagent. Highly water soluble. Working pH range 7 - 9. Used in buffer solutions such as Tris-buffered saline (TBS), Tris-acetate-EDTA (TAE) in combination with acetic acid and EDTA (Cat. No. 2811) and Tris-borate-EDTA in combination with boric acid and EDTA.

Technical Data for TRIS base

M. Wt 121.14
Formula C4H11NO3
Storage Store at RT
Purity ≥99.5%
CAS Number 77-86-1
PubChem ID 6503
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Smiles OCC(CO)(N)CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for TRIS base

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 363.42 3000

Preparing Stock Solutions for TRIS base

The following data is based on the product molecular weight 121.14. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
30 mM 0.28 mL 1.38 mL 2.75 mL
150 mM 0.06 mL 0.28 mL 0.55 mL
300 mM 0.03 mL 0.14 mL 0.28 mL
1500 mM 0.01 mL 0.03 mL 0.06 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets for TRIS base

Certificate of Analysis / Product Datasheet
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References for TRIS base

References are publications that support the biological activity of the product.

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Keywords: TRIS base, TRIS base supplier, Commonly, used, laboratory, reagent, buffer, TBS, tris, buffered, saline, TAE, TBA, acetate, EDTA, borate, Buffers,, Solvents, and, Solutions, 3163, Tocris Bioscience

Citations for TRIS base

Citations are publications that use Tocris products.

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Reviews for TRIS base

Average Rating: 5 (Based on 2 Reviews.)

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Western blot.
By Anonymous on 07/28/2018
Assay Type: In Vitro
Species: Other

We have used this product to make western blot gels of different percentages of acrylamide


Works as expected.
By Anonymous on 01/04/2018
Species: Human

I used this for making TBS buffers for western blots. All seemed to work fine.