Tricyclic cytosine tC

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Description: Fluorescent tricyclic cytosine analog for RNA imaging
Chemical Name: 3-β-D-Ribofuranosyl-1H-pyrimido[5,4-b][1,4]benzothiazin-2(3H)-one
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Tricyclic cytosine tC

Tricyclic cytosine tC is a fluorescent cytosine analog for imaging RNA in living cells. Enables the investigation of RNA synthesis, degradation, and trafficking at single-cell resolution when combined with confocal imaging. Excitation/emission maxima (λ) = 375/505 nm; extinction coefficient = 4000 M-1 cm-1; quantum yield = 0.2.

Optical Data for Tricyclic cytosine tC

Tricyclic cytosine tC Dye Spectra
λabs 375 nm
λem 505 nm
Extinction Coefficient (ε) 4000 M-1cm-1
Quantum Yield (φ) 0.2
Application Confocal Imaging

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Technical Data for Tricyclic cytosine tC

M. Wt 349.36
Formula C15H15N3O5S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1174063-74-1
Smiles O[C@H]1[C@H](N2C(N=C3C(SC4=CC=CC=C4N3)=C2)=O)O[C@@H]([C@H]1O)CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Tricyclic cytosine tC

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 34.94 100

Preparing Stock Solutions for Tricyclic cytosine tC

The following data is based on the product molecular weight 349.36. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.86 mL 14.31 mL 28.62 mL
5 mM 0.57 mL 2.86 mL 5.72 mL
10 mM 0.29 mL 1.43 mL 2.86 mL
50 mM 0.06 mL 0.29 mL 0.57 mL

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Product Datasheets for Tricyclic cytosine tC

References for Tricyclic cytosine tC

References are publications that support the biological activity of the product.

Wang et al (2022) Live-cell RNA imaging with metabolically incorporated fluorescent nucleosides. J.Am.Chem.Soc. 144 14647 PMID: 35930766

Stengel et al (2010) Incorporation of the fluorescent ribonucleotide analogue tCTP by T7 RNA polymerase. Anal.Chem. 82 1082 PMID: 20067253

Sandin et al (2005) Fluorescent properties of DNA base analogue tC upon incorporation into DNA--negligible influence of neighbouring bases on fluorescence quantum yield. Nucleic Acids Res. 33 5019 PMID: 16147985

If you know of a relevant reference for Tricyclic cytosine tC, please let us know.

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
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  • Fluorescent Probes for Imaging Bacteria
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