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Cat.No. 5227 - Tricine | C6H13NO5 | CAS No. 5704-04-1
Description: Electrophoresis and SDS-PAGE buffer
Chemical Name: N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine
Purity: ≥99%
Citations (1)
Reviews (1)
Literature (1)

Biological Activity

Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.

Technical Data

M. Wt 179.17
Formula C6H13NO5
Storage Store at RT
Purity ≥99%
CAS Number 5704-04-1
PubChem ID 79784
Smiles OCC(CO)(CO)NCC(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 17.92 100

Preparing Stock Solutions

The following data is based on the product molecular weight 179.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.58 mL 27.91 mL 55.81 mL
5 mM 1.12 mL 5.58 mL 11.16 mL
10 mM 0.56 mL 2.79 mL 5.58 mL
50 mM 0.11 mL 0.56 mL 1.12 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References are publications that support the biological activity of the product.

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1 Citation for Tricine

Citations are publications that use Tocris products. Selected citations for Tricine include:

Andrade-Talavera et al (2016) Presynaptic Spike Timing-Dependent Long-Term Depression in the Mouse Hippocampus. Cereb Cortex 26 3637 PMID: 27282393

Do you know of a great paper that uses Tricine from Tocris? Please let us know.

Reviews for Tricine

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Tris-glycine protein gel produced using Tricine.
By Anonymous on 02/06/2019
Species: Human

Tris-glycine gel formation for electrophoresis carried out by tricine. Gives better resolution for low molecular weight protein. Highly suggest using this buffer for separation of proteins that are small.

review image

Literature in this Area

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