Thioflavin T

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Description: Fluorescent amyloid stain, binds to the stacked β sheets of amyloid fibrils
Chemical Name: 2-[4-(Dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Thioflavin T

Thioflavin T is a cell-permeable fluorescent amyloid stain. This cationic benzothiazole dye increases in fluorescence upon binding to the stacked β sheets of amyloid fibrils. Thioflavin T is used to confirm formation of β sheet structure from mutant huntingtin exon-1 aggregates (Ex1Q48) in vitro. Thioflavin T is also used to monitor the kinetics of in vitro polyglutamine amyloid formation of tNhtt-42Q aggregates in a Huntington's disease cell model. Insoluble senile plaques of Aβ in brain tissues are commonly stained with Thioflavin T. Excitation/emission maxima = 440/485 nm.

This product is supplied with a high degree of hydration and some residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Optical Data for Thioflavin T

Emission Color Blue
λabs 440 nm
λem 485 nm
Extinction Coefficient (ε) 31600 M-1cm-1
Quantum Yield (φ) 0.43
Cell Permeable Yes
Application Amyloid Stain

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Technical Data for Thioflavin T

M. Wt 318.86
Formula C17H19ClN2S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2390-54-7
PubChem ID 16953
Smiles CC1=CC2=C([N+](C)=C(C3=CC=C(N(C)C)C=C3)S2)C=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Thioflavin T

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 1.59 5
water 3.19 10

Preparing Stock Solutions for Thioflavin T

The following data is based on the product molecular weight 318.86. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 31.36 mL 156.81 mL 313.62 mL
0.5 mM 6.27 mL 31.36 mL 62.72 mL
1 mM 3.14 mL 15.68 mL 31.36 mL
5 mM 0.63 mL 3.14 mL 6.27 mL

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References for Thioflavin T

References are publications that support the biological activity of the product.

Doi et al (2008) RNA-binding protein TLS is a major nuclear aggregate-interacting protein in huntingtin exon 1 with expanded polyglutamine-expressing cells. J.Biol.Chem. 283 6489 PMID: 18167354

Lee et al (2019) Fluorescence chemicals to detect insoluble and soluble amyloid-β aggregates. ACS Chem.Neurosci. 10 2647 PMID: 31009195

Schindler (2021) Small, seeding-competent huntingtin fibrils are prominent aggregate species in brains of zQ175 Huntington's disease knock-in mice. Front.Neurosci. 15 682172 PMID: 34239412

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Keywords: Thioflavin T, Thioflavin T supplier, thioflavint, cell-permeable, fluorescent, amyloid, stain, cationic, benzothiazole, dye, stacked, beta, sheets, fibrils, Fluorescent, Amyloid, B, Probes, 7122, Tocris Bioscience

Citations for Thioflavin T

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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