TBB

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Cat.No. 2275 - TBB | C6HBr4N3 | CAS No. 17374-26-4
Description: Selective cell-permeable CK2 inhibitor
Alternative Names: NSC 231634
Chemical Name: 4,5,6,7-Tetrabromobenzotriazole
Purity: ≥99% (HPLC)
Datasheet
Citations (6)
Reviews
Literature (3)

Biological Activity

A cell-permeable, selective inhibitor of casein kinase-2 (CK2) (IC50 = 0.9 and 1.6 μM for rat liver and human recombinant CK2 respectively). Exhibits modest discrimination between CK2 subunits, with Ki values ranging from 80 nM to 210 nM. Acts in an ATP/GTP-competitive manner and displays one to two orders of magnitude selectivity over a panel of 33 protein kinases.

Compound Libraries

TBB is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 434.71
Formula C6HBr4N3
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 17374-26-4
PubChem ID 1694
InChI Key OMZYUVOATZSGJY-UHFFFAOYSA-N
Smiles BrC1=C2NN=NC2=C(Br)C(Br)=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 43.47 100
ethanol 6.52 15

Preparing Stock Solutions

The following data is based on the product molecular weight 434.71. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.3 mL 11.5 mL 23 mL
5 mM 0.46 mL 2.3 mL 4.6 mL
10 mM 0.23 mL 1.15 mL 2.3 mL
50 mM 0.05 mL 0.23 mL 0.46 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Sarno et al (2001) Selectivity of 4,5,6,7-tetrabromobenzotriazole, an ATP site-directed inhibitor of protein kinase CK2 ('casein kinase-2'). FEBS Lett. 496 44 PMID: 11343704

Pagano et al (2004) Optimization of protein kinase CK2 inhibitors derived from 4,5,6,7-tetrabromobenzimidazole. J.Med.Chem. 47 6239 PMID: 15566294

Zien et al (2005) Tetrabromobenzotriazole (TBBt) and tetrabromobenzimidazole (TBBz) as selective inhibitors of protein kinase CK2: evaluation of their effects on cells and different molecular forms of human CK2. Biochim.Biophys.Acta. 1754 271 PMID: 16203192


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View all Casein Kinase 2 Inhibitors

Keywords: TBB, TBB supplier, Selective, cell-permeable, CK2, inhibitors, inhibits, Casein, Kinase, 2, Kinases, NSC231634, NSC, 231634, 2275, Tocris Bioscience

6 Citations for TBB

Citations are publications that use Tocris products. Selected citations for TBB include:

Wu et al (2014) The BCL2L1 and PGAM5 axis defines hypoxia-induced receptor-mediated mitophagy. Autophagy 10 1712 PMID: 25126723

Bartley et al (2016) Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP. Eneuro 3 PMID: 27957526

Korb et al (2015) BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice. Sci Rep 18 1464 PMID: 26301327

Navasa et al (2015) Ikaros mediates the DNA methylation-independent silencing of MCJ/DNAJC15 gene expression in macrophages. J Urol 5 14692 PMID: 26419808


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Reviews for TBB

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Literature in this Area

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