Competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C); promotes Cdc20 autoubiquitination. Inhibits cyclin proteolysis in mitotic Xenopus oocyte extracts (IC50 = 12 μM) and blocks cyclin degradation in interphase extract.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 378.87. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.64 mL||13.2 mL||26.39 mL|
|5 mM||0.53 mL||2.64 mL||5.28 mL|
|10 mM||0.26 mL||1.32 mL||2.64 mL|
|50 mM||0.05 mL||0.26 mL||0.53 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Zeng et al (2010) Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. Cancer Cell. 18 382 PMID: 20951947
Zeng & King (2012) An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat.Chem.Biol. 8 383 PMID: 22366722
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Keywords: ubiquitin ligase anaphase-promoting complex/cyclosome APC inhibits inhibitors competitive mitotic arrest cyclin stabilization TAME hydrochloride HCl Ubiquitin E3 Ligases
1 Citation for TAME hydrochloride
Citations are publications that use Tocris products. Selected citations for TAME hydrochloride include:
Coles et al (2015) KIF7 Controls the Proliferation of Cells of the Respiratory Airway through Distinct Microtubule Dependent Mechanisms. PLoS Genet 11 e1005525 PMID: 26439735
Do you know of a great paper that uses TAME hydrochloride from Tocris? If so please let us know.
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