Sulfo-NHS-LC-Biotin

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Cat.No. 7302 - Sulfo-NHS-LC-Biotin | C20H29N4NaO9S2 | CAS No. 191671-46-2
Description: Amine reactive biotinylation reagent, cell impermeable
Chemical Name: Sodium 2,5-dioxo-1-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)pyrrolidine-3-sulfonate
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.

Technical Data

M. Wt 556.58
Formula C20H29N4NaO9S2
Storage Store at -20°C
CAS Number 191671-46-2
PubChem ID 71571497
InChI Key JJGWLCLUQNFDIS-GTSONSFRSA-M
Smiles [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCC(ON3C(CC(S([O-])(=O)=O)C3=O)=O)=O)=O.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 55.66 100
DMSO 55.66 100

Preparing Stock Solutions

The following data is based on the product molecular weight 556.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.8 mL 8.98 mL 17.97 mL
5 mM 0.36 mL 1.8 mL 3.59 mL
10 mM 0.18 mL 0.9 mL 1.8 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Takayama et al (2017) Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method. Sci.Rep 7 16953 PMID: 29208980

Xu and Wegner (2020) Multifunctional streptavidin-biotin conjugates with precise stoichiometries. Chem.Sci. 11 4422

Karunakaran et al (2020) Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing. Immunity 52 487 PMID: 32155411


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Keywords: Sulfo-NHS-LC-Biotin, Sulfo-NHS-LC-Biotin supplier, Water, soluble, biotinylation, reagents, primary, amine, labels, Biochemicals, and, Molecular, Biology, 7302, Tocris Bioscience

Citations for Sulfo-NHS-LC-Biotin

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