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Description: Amine reactive biotinylation reagent, cell impermeable
Chemical Name: Sodium 2,5-dioxo-1-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)pyrrolidine-3-sulfonate
Citations (1)

Biological Activity for Sulfo-NHS-LC-Biotin

Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.

Technical Data for Sulfo-NHS-LC-Biotin

M. Wt 556.58
Formula C20H29N4NaO9S2
Storage Store at -20°C
CAS Number 191671-46-2
PubChem ID 71571497
Smiles [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCC(ON3C(CC(S([O-])(=O)=O)C3=O)=O)=O)=O.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Sulfo-NHS-LC-Biotin

Solvent Max Conc. mg/mL Max Conc. mM
water 55.66 100
DMSO 55.66 100

Preparing Stock Solutions for Sulfo-NHS-LC-Biotin

The following data is based on the product molecular weight 556.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.8 mL 8.98 mL 17.97 mL
5 mM 0.36 mL 1.8 mL 3.59 mL
10 mM 0.18 mL 0.9 mL 1.8 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

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Product Datasheets for Sulfo-NHS-LC-Biotin

Certificate of Analysis / Product Datasheet
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References for Sulfo-NHS-LC-Biotin

References are publications that support the biological activity of the product.

Takayama et al (2017) Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method. Sci.Rep 7 16953 PMID: 29208980

Xu and Wegner (2020) Multifunctional streptavidin-biotin conjugates with precise stoichiometries. Chem.Sci. 11 4422

Karunakaran et al (2020) Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing. Immunity 52 487 PMID: 32155411

If you know of a relevant reference for Sulfo-NHS-LC-Biotin, please let us know.

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Keywords: Sulfo-NHS-LC-Biotin, Sulfo-NHS-LC-Biotin supplier, Water, soluble, biotinylation, reagents, primary, amine, labels, Biochemicals, and, Molecular, Biology, 7302, Tocris Bioscience

1 Citation for Sulfo-NHS-LC-Biotin

Citations are publications that use Tocris products. Selected citations for Sulfo-NHS-LC-Biotin include:

Andrei I et al (2023) Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2. iScience 26 106195 PMID: 36890793

Do you know of a great paper that uses Sulfo-NHS-LC-Biotin from Tocris? Please let us know.

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