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Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 556.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.8 mL||8.98 mL||17.97 mL|
|5 mM||0.36 mL||1.8 mL||3.59 mL|
|10 mM||0.18 mL||0.9 mL||1.8 mL|
|50 mM||0.04 mL||0.18 mL||0.36 mL|
References are publications that support the biological activity of the product.
Takayama et al (2017) Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method. Sci.Rep 7 16953 PMID: 29208980
Xu and Wegner (2020) Multifunctional streptavidin-biotin conjugates with precise stoichiometries. Chem.Sci. 11 4422
Karunakaran et al (2020) Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing. Immunity 52 487 PMID: 32155411
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Keywords: Sulfo-NHS-LC-Biotin, Sulfo-NHS-LC-Biotin supplier, Water, soluble, biotinylation, reagents, primary, amine, labels, Biochemicals, and, Molecular, Biology, 7302, Tocris Bioscience
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