Sulfidefluor 7 AM

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Description: H2S fluorescent probe
Chemical Name: N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[(3',6'-diazido-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]glycine (acetyloxy)methyl ester
Citations (4)
Literature (1)

Biological Activity for Sulfidefluor 7 AM

Sulfidefluor 7 AM is a reported to be a hydrogen sulphide (H2S) fluorescent probe. Contains an acetoxymethyl ester, which enhances cell permeability and is hydrolyzed inside the cell to trap the probe. Used to visualize VEGF-induced H2S production in human umbilical vein endothelial cells (HUVECS). Excitation wavelength ~ 495 nm; emission maximum ~ 520 nm.

Licensing Information

Sold under license from the Regents of the University of California

Optical Data for Sulfidefluor 7 AM

Emission Color Green
λabs 495 nm
λem 520 nm
Cell Permeable Yes
Application Fluorescence Microscopy

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Technical Data for Sulfidefluor 7 AM

M. Wt 685.55
Formula C31H23N7O12
Storage Store at -20°C
CAS Number 1416872-50-8
PubChem ID 89306941
Smiles O=C(O1)C2=C(C=CC(C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O)=C2)C31C4=CC=C(N=[N+]=[N-])C=C4OC5=C3C=CC(N=[N+]=[N-])=C5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Sulfidefluor 7 AM

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 13.71 20

Preparing Stock Solutions for Sulfidefluor 7 AM

The following data is based on the product molecular weight 685.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.29 mL 36.47 mL 72.93 mL
1 mM 1.46 mL 7.29 mL 14.59 mL
2 mM 0.73 mL 3.65 mL 7.29 mL
10 mM 0.15 mL 0.73 mL 1.46 mL

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Product Datasheets for Sulfidefluor 7 AM

Certificate of Analysis / Product Datasheet
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References for Sulfidefluor 7 AM

References are publications that support the biological activity of the product.

Lin et al (2013) Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production. Proc.Natl.Acad.Sci.USA. 110 7131 PMID: 23589874

Lippert et al (2011) Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells. J.Am.Chem.Soc. 133 10078 PMID: 21671682

If you know of a relevant reference for Sulfidefluor 7 AM, please let us know.

Keywords: Sulfidefluor 7 AM, Sulfidefluor 7 AM supplier, hydrogen, sulphide, H2S, fluorescent, probe, Fluorescent, Cell, Indicators, and, Sensors, 4943, Tocris Bioscience

4 Citations for Sulfidefluor 7 AM

Citations are publications that use Tocris products. Selected citations for Sulfidefluor 7 AM include:

Chhabra et al (2018) Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4 6 PMID: 29531803

Xiaofeng et al (2021) Intracellular H2S production is an autophagy-dependent adaptive response to DNA damage. Cell Chem Biol 28 1669-1678.e5 PMID: 34166610

Cheng et al (2016) Restoration of Hydrogen Sulfide Production in Diabetic Mice Improves Reparative Function of Bone Marrow Cells. Circulation 134 1467 PMID: 27660293

Margreet R et al (2020) Periprocedural Hydrogen Sulfide Therapy Improves Vascular Remodeling and Attenuates Vein Graft Disease. J Am Heart Assoc 9 e016391 PMID: 33146045

Do you know of a great paper that uses Sulfidefluor 7 AM from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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