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Sulfidefluor 7 AM
Reported to be a hydrogen sulphide (H2S) fluorescent probe. Contains an acetoxymethyl ester, which enhances cell permeability and is hydrolyzed inside the cell to trap the probe. Used to visualize VEGF-induced H2S production in human umbilical vein endothelial cells (HUVECS). Excitation wavelength ~ 495 nm; emission maximum ~ 520 nm.
Sold under license from the Regents of the University of California
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 685.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||7.29 mL||36.47 mL||72.93 mL|
|1 mM||1.46 mL||7.29 mL||14.59 mL|
|2 mM||0.73 mL||3.65 mL||7.29 mL|
|10 mM||0.15 mL||0.73 mL||1.46 mL|
References are publications that support the biological activity of the product.
Lin et al (2013) Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production. Proc.Natl.Acad.Sci.USA. 110 7131 PMID: 23589874
Lippert et al (2011) Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells. J.Am.Chem.Soc. 133 10078 PMID: 21671682
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Keywords: Sulfidefluor 7 AM, Sulfidefluor 7 AM supplier, hydrogen, sulphide, H2S, fluorescent, probe, Fluorescent, Cell, Indicators, and, Sensors, 4943, Tocris Bioscience
2 Citations for Sulfidefluor 7 AM
Citations are publications that use Tocris products. Selected citations for Sulfidefluor 7 AM include:
Chhabra et al (2018) Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4 6 PMID: 29531803
Cheng et al (2016) Restoration of Hydrogen Sulfide Production in Diabetic Mice Improves Reparative Function of Bone Marrow Cells. Circulation 134 1467 PMID: 27660293
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