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Commonly used laboratory reagent
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 58.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|5 mM||3.42 mL||17.11 mL||34.22 mL|
|25 mM||0.68 mL||3.42 mL||6.84 mL|
|50 mM||0.34 mL||1.71 mL||3.42 mL|
|250 mM||0.07 mL||0.34 mL||0.68 mL|
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NaCl (137 mM) is added in the extracellular buffer, consisting of (in mM) 5.4 KCl, 1 CaCl2, 1 MgCl2, 10 HEPES and 10 glucose and pH is adjusted to 7.4 with NaOH and used to perfuse cells during Ca2+ imaging in cells.