SCR7 pyrazine

Pricing Availability Delivery Time Qty
Cat.No. 5342 - SCR7 pyrazine | C18H12N4OS | CAS No. 14892-97-8
Description: Enhances HDR efficiency
Chemical Name: 2,3-Dihydro-6,7-diphenyl-2-thioxo-4(1H)-pteridinone
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).

We have discovered that the commercially available SCR7 material supplied by other vendors does not match the chemical structure that it is being sold under. Through detailed chemical analysis carried out by our Analytical Quality Control experts, we believe that the commercially available SCR7 material, including that used in recent publications to enhance CRISPR efficiency, is in fact an analog of SCR7 that we have named 'SCR7 pyrazine'.

View more information regarding the structure of SCR7.

Technical Data

M. Wt 332.38
Formula C18H12N4OS
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 14892-97-8
PubChem ID 10688007
InChI Key GSRTWXVBHGOUBU-UHFFFAOYSA-N
Smiles O=C(C(N=C(C3=CC=CC=C3)C(C4=CC=CC=C4)=N2)=C2N1)NC1=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 33.44 100
ethanol 6.69 20

Preparing Stock Solutions

The following data is based on the product molecular weight 332.38. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.01 mL 15.04 mL 30.09 mL
5 mM 0.6 mL 3.01 mL 6.02 mL
10 mM 0.3 mL 1.5 mL 3.01 mL
50 mM 0.06 mL 0.3 mL 0.6 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Chu et al (2015) Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat.Biotechnol. 33 543 PMID: 25803306

Maruyama et al (2015) Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat.Biotechnol. 33 538 PMID: 25798939

Greco et al (2016) Synthesis and structure determination of SCR7, a DNA ligase inhibitor Teterahedron Lett. 57 3204 PMID:

Greco et al (2016) SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV. DNA Repair (Amst) 43 18 PMID: 27235626


If you know of a relevant reference for SCR7 pyrazine, please let us know.

View Related Products by Target

Keywords: CRISPR Cas9 HDR NHEJ homology directed repair nonhomologous end joining CRISPR Reagents

Citations for SCR7 pyrazine

Citations are publications that use Tocris products.

Currently there are no citations for SCR7 pyrazine. Do you know of a great paper that uses SCR7 pyrazine from Tocris? If so please let us know.

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Literature in this Area

New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

Pathways for SCR7 pyrazine

Protocols

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