SCR7 pyrazine

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Cat.No. 5342 - SCR7 pyrazine | C18H12N4OS | CAS No. 14892-97-8
Description: Enhances HDR efficiency
Chemical Name: 2,3-Dihydro-6,7-diphenyl-2-thioxo-4(1H)-pteridinone
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).

We have discovered that the commercially available SCR7 material supplied by other vendors does not match the chemical structure that it is being sold under. Through detailed chemical analysis carried out by our Analytical Quality Control experts, we believe that the commercially available SCR7 material, including that used in recent publications to enhance CRISPR efficiency, is in fact an analog of SCR7 that we have named 'SCR7 pyrazine'.

View more information regarding the structure of SCR7.

Technical Data

M. Wt 332.38
Formula C18H12N4OS
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 14892-97-8
PubChem ID 10688007
InChI Key GSRTWXVBHGOUBU-UHFFFAOYSA-N
Smiles O=C(C(N=C(C3=CC=CC=C3)C(C4=CC=CC=C4)=N2)=C2N1)NC1=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 33.44 100
ethanol 6.69 20

Preparing Stock Solutions

The following data is based on the product molecular weight 332.38. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.01 mL 15.04 mL 30.09 mL
5 mM 0.6 mL 3.01 mL 6.02 mL
10 mM 0.3 mL 1.5 mL 3.01 mL
50 mM 0.06 mL 0.3 mL 0.6 mL

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References

References are publications that support the biological activity of the product.

Chu et al (2015) Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat.Biotechnol. 33 543 PMID: 25803306

Maruyama et al (2015) Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat.Biotechnol. 33 538 PMID: 25798939

Greco et al (2016) Synthesis and structure determination of SCR7, a DNA ligase inhibitor Teterahedron Lett. 57 3204

Greco et al (2016) SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV. DNA Repair (Amst) 43 18 PMID: 27235626


If you know of a relevant reference for SCR7 pyrazine, please let us know.

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Keywords: SCR7 pyrazine, SCR7 pyrazine supplier, CRISPR, Cas9, HDR, NHEJ, homology, directed, repair, nonhomologous, end, joining, Reagents, 5342, Tocris Bioscience

Citations for SCR7 pyrazine

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Literature in this Area

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