Enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).
We have discovered that the commercially available SCR7 material supplied by other vendors does not match the chemical structure that it is being sold under. Through detailed chemical analysis carried out by our Analytical Quality Control experts, we believe that the commercially available SCR7 material, including that used in recent publications to enhance CRISPR efficiency, is in fact an analog of SCR7 that we have named 'SCR7 pyrazine'.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 332.38. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.01 mL||15.04 mL||30.09 mL|
|5 mM||0.6 mL||3.01 mL||6.02 mL|
|10 mM||0.3 mL||1.5 mL||3.01 mL|
|50 mM||0.06 mL||0.3 mL||0.6 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Chu et al (2015) Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat.Biotechnol. 33 543 PMID: 25803306
Maruyama et al (2015) Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat.Biotechnol. 33 538 PMID: 25798939
Greco et al (2016) SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV. DNA Repair (Amst) 43 18 PMID: 27235626
If you know of a relevant reference for SCR7 pyrazine, please let us know.
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Keywords: CRISPR Cas9 HDR NHEJ homology directed repair nonhomologous end joining CRISPR Reagents
Citations for SCR7 pyrazine
Citations are publications that use Tocris products.
Currently there are no citations for SCR7 pyrazine. Do you know of a great paper that uses SCR7 pyrazine from Tocris? If so please let us know.
Literature in this Area
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