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Submit ReviewSCOTfluor lactic acid probe 510 is a red fluorescent probe for imaging lactic acid metabolism in vivo. Excitation and emission maxima (λ) are 485 and 605 nm, respectively. Suitable for use with flow cytometry and super-resolution microscopy.
Application of SCOTfluor lactic acid probe 510 in HeLa cells. TIRF microscopy image (left) of HeLa cells after incubation with SCOTfluor 510 lactate (100 μM). Multi-tracking of fluorescent particles corresponding to SCOTfluor 510 lactate labelling (right). Scale: 5 μm. Image kindly provided by Prof. Marc Vendrell, acquired by Dr Mathew Horrocks, School of Chemistry, University of Edinburgh.
Sold under license from the University of Edinburgh
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral ViewerM. Wt | 331.15 |
Formula | C9H8N4O5Se |
Storage | Store at -20°C |
Purity | ≥95% (HPLC) |
CAS Number | 2351933-31-6 |
PubChem ID | 146168509 |
InChI Key | HQRYGRFCRSBEJR-LURJTMIESA-N |
Smiles | O[C@H](C(O)=O)CNC1=CC=C(N(=O)=O)C2=N[Se]N=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
DMSO | 3.31 | 10 |
The following data is based on the product molecular weight 331.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.1 mM | 30.2 mL | 150.99 mL | 301.98 mL |
0.5 mM | 6.04 mL | 30.2 mL | 60.4 mL |
1 mM | 3.02 mL | 15.1 mL | 30.2 mL |
5 mM | 0.6 mL | 3.02 mL | 6.04 mL |
References are publications that support the biological activity of the product.
Benson et al (2019) SCOTfluors: Small, conjugatable, orthogonal, and tunable fluorophores for in vivo imaging of cell metabolism. Angew.Chem Int.Ed.Engl. 58 6911 PMID: 30924239
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Keywords: SCOTfluor lactic acid probe 510, SCOTfluor lactic acid probe 510 supplier, metabolism, imaging, lactic, acid, fluorophore, fluorescent, in, vivo, Other, Fluorescent, Probes, 7448, Tocris Bioscience
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