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Submit ReviewSCOTfluor lactic acid probe 510
Description: Fluorescent lactic acid probe
Chemical Name: (2S)-2-Hydroxy-3-[(7-nitro-2,1,3-benzoselenadiazol-4-yl)amino]propanoic acid
Purity: ≥95% (HPLC)
Biological Activity for SCOTfluor lactic acid probe 510
SCOTfluor lactic acid probe 510 is a red fluorescent probe for imaging lactic acid metabolism in vivo. Excitation and emission maxima (λ) are 485 and 605 nm, respectively. Suitable for use with flow cytometry and super-resolution microscopy.
Scientific Data
Fluorescence Imaging
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Application of SCOTfluor lactic acid probe 510 in HeLa cells. TIRF microscopy image (left) of HeLa cells after incubation with SCOTfluor 510 lactate (100 μM). Multi-tracking of fluorescent particles corresponding to SCOTfluor 510 lactate labelling (right). Scale: 5 μm. Image kindly provided by Prof. Marc Vendrell, acquired by Dr Mathew Horrocks, School of Chemistry, University of Edinburgh.
Licensing Information
Sold under license from the University of Edinburgh
Optical Data for SCOTfluor lactic acid probe 510
| Emission Color | Orange |
|---|---|
| λabs | 485 nm |
| λem | 605 nm |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy, Super Resolution Microscopy (SRM), Flow Cytometry, in vivo |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for SCOTfluor lactic acid probe 510
| M. Wt | 331.15 |
| Formula | C9H8N4O5Se |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 2351933-31-6 |
| PubChem ID | 146168509 |
| InChI Key | HQRYGRFCRSBEJR-LURJTMIESA-N |
| Smiles | O[C@H](C(O)=O)CNC1=CC=C(N(=O)=O)C2=N[Se]N=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SCOTfluor lactic acid probe 510
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 3.31 | 10 |
Preparing Stock Solutions for SCOTfluor lactic acid probe 510
The following data is based on the product molecular weight 331.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 30.2 mL | 150.99 mL | 301.98 mL |
| 0.5 mM | 6.04 mL | 30.2 mL | 60.4 mL |
| 1 mM | 3.02 mL | 15.1 mL | 30.2 mL |
| 5 mM | 0.6 mL | 3.02 mL | 6.04 mL |
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Product Datasheets for SCOTfluor lactic acid probe 510
References for SCOTfluor lactic acid probe 510
References are publications that support the biological activity of the product.
Benson et al (2019) SCOTfluors: Small, conjugatable, orthogonal, and tunable fluorophores for in vivo imaging of cell metabolism. Angew.Chem Int.Ed.Engl. 58 6911 PMID: 30924239
If you know of a relevant reference for SCOTfluor lactic acid probe 510, please let us know.
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Keywords: SCOTfluor lactic acid probe 510, SCOTfluor lactic acid probe 510 supplier, metabolism, imaging, lactic, acid, fluorophore, fluorescent, in, vivo, Other, Fluorescent, Probes, 7448, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
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