SAG dihydrochloride

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Description: Dihydrochloride salt of SAG; Smo receptor agonist
Chemical Name: 3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide dihydrochloride
Purity: ≥98% (HPLC)
Citations (3)
Reviews (1)
Literature (1)

Biological Activity for SAG dihydrochloride

SAG dihydrochloride is a water-soluble dihydrochloride salt of SAG (Cat. No. 4366). SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons. The compound can be used in protocols for the chemical reprogramming of somatic cells to iPSCs.

Compound Libraries for SAG dihydrochloride

SAG dihydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SAG dihydrochloride

M. Wt 562.98
Formula C28H28ClN3OS.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2702366-44-5
PubChem ID 71433770
Smiles ClC1=C(C(N([C@H]2CC[C@H](NC)CC2)CC3=CC=CC(C4=CC=NC=C4)=C3)=O)SC5=CC=CC=C51.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SAG dihydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 56.3 100
DMSO 56.3 100

Preparing Stock Solutions for SAG dihydrochloride

The following data is based on the product molecular weight 562.98. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.78 mL 8.88 mL 17.76 mL
5 mM 0.36 mL 1.78 mL 3.55 mL
10 mM 0.18 mL 0.89 mL 1.78 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

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Product Datasheets for SAG dihydrochloride

Certificate of Analysis / Product Datasheet
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References for SAG dihydrochloride

References are publications that support the biological activity of the product.

Guan et al (2022) Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605 325 PMID: 35418683

If you know of a relevant reference for SAG dihydrochloride, please let us know.

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Keywords: SAG dihydrochloride, SAG dihydrochloride supplier, Potent, Smoothened, Smo, receptors, agonists, activates, Hedgehog, signaling, pathway, activator, enhancers, neuronal, differentiation, iPSCs, induced, pluripotent, stem, cells, dopaminergic, neurons, neurones, 912545-86-9, reprogramming, Receptors, Neural, Stem, Cells, Cell, Reprogramming, 6390, Tocris Bioscience

3 Citations for SAG dihydrochloride

Citations are publications that use Tocris products. Selected citations for SAG dihydrochloride include:

Lucia et al (2020) HDL inhibits endoplasmic reticulum stress-induced apoptosis of pancreatic β-cells in vitro by activation of Smoothened. J Lipid Res 61 492-504 PMID: 31907205

Madeline A et al (2021) Population-scale single-cell RNA-seq profiling across dopaminergic neuron differentiation. Nat Genet 53 304-312 PMID: 33664506

Joaquin M et al (2018) Trisomy 21 Represses Cilia Formation and Function. Dev Cell 46 641-650.e6 PMID: 30100262

Do you know of a great paper that uses SAG dihydrochloride from Tocris? Please let us know.

Reviews for SAG dihydrochloride

Average Rating: 5 (Based on 1 Review.)

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MUCH better than the others!!.
By Anonymous on 08/28/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: stem cells

This SAG performs the BEST for stem cell differentiation to neuronal lineages. Other brands do not work. We use it at 1uM after dual SMAD inhibition.

Literature in this Area

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Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
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