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Cat.No. 1852 - (S)-(-)-Blebbistatin | C18H16N2O2 | CAS No. 856925-71-8
Description: Selective inhibitor of myosin II ATPase activity. Active enantiomer of (±)-blebbistatin (Cat. No. 1760)
Chemical Name: (3aS)-(-)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Purity: ≥99% (HPLC)
Citations (2)

Biological Activity

Selective inhibitor of myosin II ATPase activity (IC50 ~0.5 - 5 μM); active enantiomer of (±)-blebbistatin. Inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells.

Inactive Enantiomer and Racemate also available.

Technical Data

M. Wt 292.34
Formula C18H16N2O2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 856925-71-8
PubChem ID 5287792
Smiles O=C2[C@@]3(O)C(N(C4=CC=CC=C4)CC3)=NC1=CC=C(C)C=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 29.23 100

Preparing Stock Solutions

The following data is based on the product molecular weight 292.34. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.42 mL 17.1 mL 34.21 mL
5 mM 0.68 mL 3.42 mL 6.84 mL
10 mM 0.34 mL 1.71 mL 3.42 mL
50 mM 0.07 mL 0.34 mL 0.68 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References are publications that support the products' biological activity.

Limouze et al (2004) Specificity of blebbistatin, an inhibitor of myosin II. J.Muscle Res.Cell Motil. 25 337 PMID: 15548862

Straight et al (2003) Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299 1743 PMID: 12637748

Straight et al (2002) Dissecting cytokinesis with small molecules. Amer.Soc.Cell Biol.Abstr. 2515 PMID:

If you know of a relevant reference for (S)-(-)-Blebbistatin, please let us know.

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Keywords: (S)-(-)-Blebbistatin, supplier, Selective, inhibitors, inhibits, myosin, II, ATPase, Myosin, Myosin, Tocris Bioscience

2 Citations for (S)-(-)-Blebbistatin

Citations are publications that use Tocris products. Selected citations for (S)-(-)-Blebbistatin include:

Bzymek et al (2016) Real-time two- and three-dimensional imaging of monocyte motility and navigation on planar surfaces and in collagen matrices: roles of Rho. Sci Rep 6 25016 PMID: 27122054

Maître et al (2016) Asymmetric division of contractile domains couples cell positioning and fate specification. Nature 536 344 PMID: 27487217

Do you know of a great paper that uses (S)-(-)-Blebbistatin from Tocris? If so please let us know.

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