(±)-Blebbistatin

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Cat.No. 1760 - (±)-Blebbistatin | C18H16N2O2 | CAS No. 674289-55-5
Description: Selective inhibitor of myosin II ATPase activity
Chemical Name: (±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Purity: ≥98% (HPLC)
Datasheet
Citations (41)
Reviews
Literature

Biological Activity

Selective inhibitor of myosin II ATPase activity; inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells.

Active and Inactive Enantiomer also available.

Compound Libraries

(±)-Blebbistatin is also offered as part of the Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 292.34
Formula C18H16N2O2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 674289-55-5
PubChem ID 3476986
InChI Key LZAXPYOBKSJSEX-UHFFFAOYSA-N
Smiles CC1=CC=C2N=C3N(CCC3(O)C(=O)C2=C1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 29.23 100

Preparing Stock Solutions

The following data is based on the product molecular weight 292.34. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.42 mL 17.1 mL 34.21 mL
5 mM 0.68 mL 3.42 mL 6.84 mL
10 mM 0.34 mL 1.71 mL 3.42 mL
50 mM 0.07 mL 0.34 mL 0.68 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the biological activity of the product.

Limouze et al (2004) Specificity of blebbistatin, an inhibitor of myosin II. J.Muscle Res.Cell Motil. 25 337 PMID: 15548862

Straight et al (2003) Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299 1743 PMID: 12637748

Straight et al (2002) Dissecting cytokinesis with small molecules. Amer.Soc.Cell Biol.Abstr. 2515

Walker et al (2010) Non-muscle myosin II regulates survival threshold of pluripotent stem cells. Nat.Commun. 1 71 PMID: 20842192


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Keywords: (±)-Blebbistatin, (±)-Blebbistatin supplier, Selective, inhibitors, inhibits, myosin, II, ATPase, activity, P-Type, V-Type, E-Type, F-Type, ATPases, GTPases, Myosin, 1760, Tocris Bioscience

41 Citations for (±)-Blebbistatin

Citations are publications that use Tocris products. Selected citations for (±)-Blebbistatin include:

Matsubayashi et al (2011) 'White wave' analysis of epithelial scratch wound healing reveals how cells mobilise back from the leading edge in a myosin-II-dependent fashion. Proc Natl Acad Sci U S A 124 1017 PMID: 21402875

Lang et al (2011) Role of Pyk2 in cardiac arrhythmogenesis. J Inflamm (Lond) 301 H975 PMID: 21666110

Rath et al (2017) ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth. EMBO Mol Med 9 198 PMID: 28031255

Talele et al (2015) Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells. Stem Cell Reports 4 1016 PMID: 26028530

Zangle et al (2014) Live Cell Interferometry Quantifies Dynamics of Biomass Partitioning during Cytokinesis. PLoS One 9 e115726 PMID: 25531652

Vidali et al (2006) Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor. Mol Biol Cell 17 2377 PMID: 16525021

Charras et al (2006) Reassembly of contractile actin cortex in cell blebs. J Cell Biol 175 477 PMID: 17088428

Gao et al (2016) MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection. Nat Commun 6 25120 PMID: 27112594

Seese et al (2012) LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice. J Neurosci 32 7403 PMID: 22623686

Burkel et al (2012) A Rho GTPase signal treadmill backs a contractile array. Dev Cell 23 384 PMID: 22819338

Chu et al (2012) Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO(i) Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway. J Signal Transduct 2012 473410 PMID: 22900166

Nelson et al (2005) Emergent patterns of growth controlled by multicellular form and mechanics. Am J Physiol Heart Circ Physiol 102 11594 PMID: 16049098

Bzymek et al (2016) Real-time two- and three-dimensional imaging of monocyte motility and navigation on planar surfaces and in collagen matrices: roles of Rho. Sci Rep 6 25016 PMID: 27122054

Olivas et al (2016) Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130. J Neurosci 36 479 PMID: 26758839

Chaudhuri et al (2015) Substrate stress relaxation regulates cell spreading. Int J Nanomedicine 6 6364 PMID: 25695512

Tai et al (2015) Simvastatin enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation. Heart Rhythm 10 5881 PMID: 26451103

Jesus et al (2015) Atherosclerosis exacerbates arrhythmia following myocardial infarction: Role of myocardial inflammation. Mol Biol Cell 12 169 PMID: 25304682

Chazeau et al (2015) Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines. Int J Cancer 26 859 PMID: 25568337

Choi et al (2015) SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II. J Cell Biol 136 E219 PMID: 25137150

Tornavaca et al (2015) ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. Cell 208 821 PMID: 25753039

Ruprecht et al (2015) Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. PLoS One 160 673 PMID: 25679761

Chang et al (2015) Role of sarcoplasmic reticulum calcium in development of secondary calcium rise and early afterdepolarizations in long QT syndrome rabbit model. J Cell Sci 10 e0123868 PMID: 25875599

Wang et al (2014) Optical mapping of sarcoplasmic reticulum Ca2+ in the intact heart: ryanodine receptor refractoriness during alternans and fibrillation. Circ Res 114 1410 PMID: 24568740

Pietka et al (2012) CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting. J Biol Chem 287 38901 PMID: 23019328

Rossy et al (2009) Flotillins interact with PSGL-1 in neutrophils and, upon stimulation, rapidly organize into membrane domains subsequently accumulating in the uropod. PLoS One 4 e5403 PMID: 19404397

Gardner et al (2015) Targeting protein tyrosine phosphatase σ after myocardial infarction restores cardiac sympathetic innervation and prevents arrhythmias. Nat Commun 6 6235 PMID: 25639594

Chabaud et al (2015) Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. Nat Commun 6 7526 PMID: 26109323

Sulkin et al (2013) Three-dimensional printing physiology laboratory technology. Am J Physiol Heart Circ Physiol 305 H1569 PMID: 24043254

Erickson et al (2013) Diabetic hyperglycaemia activates CaMKII and arrhythmias by O-linked glycosylation. Nature 502 372 PMID: 24077098

Glukhov et al (2013) Functional roles of KATP channel subunits in metabolic inhibition. J Mol Cell Cardiol 62 90 PMID: 23624089

Li et al (2013) Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation. Proc Natl Acad Sci U S A 110 E1452 PMID: 23576731

Glukhov et al (2013) Sinoatrial node reentry in a canine chronic left ventricular infarct model: role of intranodal fibrosis and heterogeneity of refractoriness. Circ Arrhythm Electrophysiol 6 984 PMID: 23960214

Liu et al (2013) Mitochondrial dysfunction causing cardiac sodium channel downregulation in cardiomyopathy. J Mol Cell Cardiol 54 25 PMID: 23123323

Haan et al (2013) Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy. PLoS One 8 e59290 PMID: 23516623

Kong et al (2012) Intramural optical mapping of V(m) and Ca(i)2+ during long-duration ventricular fibrillation in canine hearts. Am J Physiol Heart Circ Physiol 302 H1294 PMID: 22268104

Chiu et al (2012) EGFR and myosin II inhibitors cooperate to suppress EGFR-T790M-mutant NSCLC cells. Mol Oncol 6 299 PMID: 22366308

Fedorov et al (2011) Effects of KATP channel openers diazoxide and pinacidil in coronary-perfused atria and ventricles from failing and non-failing human hearts. J Mol Cell Cardiol 51 215 PMID: 21586291

Kaur et al (2011) RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers. Arterioscler Thromb Vasc Biol 31 657 PMID: 21148427

Lang et al (2011) Optical mapping of action potentials and calcium transients in the mouse heart. J Vis Exp PMID: 21946907

Lou et al (2011) Transmural heterogeneity and remodeling of ventricular excitation-contraction coupling in human heart failure. Circulation 123 1881 PMID: 21502574

Ruiz and Chen (2008) Emergence of patterned stem cell differentiation within multicellular structures. Sci Rep 26 2921 PMID: 18703661


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