Pyridostatin pentahydrochloride

Pricing Availability Delivery Time Qty
Cat.No. 4763 - Pyridostatin pentahydrochloride | C31H32N8O5.5HCl
Description: Stabilizes G-quadruplexes; induces DNA damage and cell cycle arrest
Chemical Name: 4-(2-Aminoethoxy)N2,N6-bis[(4-(2-aminoethoxy)-2-quinolinyl]-2,6-pyridinecarboxamide pentahydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Binds and stabilizes G-quadruplexes, inducing DNA damage and cell cycle arrest (Kd = 490 nM); targets the proto-oncogene Src, reducing Src protein abundance and Src-dependent motility in human breast cancer cells. Also targets telomeric G-quadruplexes, inducing telomerase dysfunction. Activates the DNA-dependent protein kinase catalytic sunbunit (DNA-PKcs).

Technical Data

M. Wt 778.94
Formula C31H32N8O5.5HCl
Storage Desiccate at RT
Purity ≥98% (HPLC)
PubChem ID 78243739
InChI Key SRIZPFGTXSQRFM-UHFFFAOYSA-N
Smiles NCCOC1=CC(C(NC2=NC(C=CC=C3)=C3C(OCCN)=C2)=O)=NC(C(NC4=CC(OCCN)=C(C=CC=C5)C5=N4)=O)=C1.Cl.Cl.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 77.89 100

Preparing Stock Solutions

The following data is based on the product molecular weight 778.94. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.28 mL 6.42 mL 12.84 mL
5 mM 0.26 mL 1.28 mL 2.57 mL
10 mM 0.13 mL 0.64 mL 1.28 mL
50 mM 0.03 mL 0.13 mL 0.26 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Rodriguez et al (20112) Small-molecule-induced DNA damage identifies alternative DNA structures in human genes. Nat.Chem.Biol. 8 301 PMID: 22306580

Müller et al (2010) Small-molecule-mediated G-quadruplex isolation from human cells. Nat.Chem. 2 1095 PMID: 21107376

Koirala et al (2011) A single-molecule platform for investigation of interactions between G-quadruplexes and small-molecule ligands. Nat.Chem. 3 782 PMID: 1941250


If you know of a relevant reference for Pyridostatin pentahydrochloride, please let us know.

Keywords: Pyridostatin 5HCl stabilizes G-quadruplexes DNA damage cell cycle arrest proto-oncogene SRC telomerase telomeric DNA-dependent protein kinase catalytic DNA-PKcs sunbunit Cell Cycle Inhibitors

Citations for Pyridostatin pentahydrochloride

Citations are publications that use Tocris products.

Currently there are no citations for Pyridostatin pentahydrochloride. Do you know of a great paper that uses Pyridostatin pentahydrochloride from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Pyridostatin pentahydrochloride

Protocols

TODO: Add Protocols